Medicni perspektivi, 2022;27(3)
2022 Vol. XXVII N 3
Sokolova K.V., Stavytskyi V.V., Voskoboinik O.Yu., Podpletnya O.A., Kovalenko S.I. In silico and in vivo screening of triamterene synthetic analogues as promising diuretics
Authors and affiliations
Stavytskyi V.V. Oles Honchar Dnipro National University, Dnipro, Ukraine
Voskoboinik O.Yu. Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
Podpletnya O.A. Dnipro State Medical University, Dnipro, Ukraine
Kovalenko S.I. Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
Key words: design, triamterene, pteridines, structural modification, molecular docking, diuretic activity, SAR analysis
Abstract. In silico and in vivo screening of triamterene synthetic analogues as promising diuretics. Sokolova K.V., Stavytskyi V.V., Voskoboinik O.Yu., Podpletnya O.A., Kovalenko S.I. The modification of lead-compound aimed to the increasing of activity, decrement of toxicity or improvement of selectivity is one of the most important methods used for elaboration of novel medications. Natural compounds, approved or investigational drugs or just compounds with proved biological activity could be the lead-compound. Often the chemical modification of lead compounds is directed at the enhancement of ligand-biological target interactions. Abovementioned approach, namely structural modification of known drug triamterene was used for purposeful search for novel diuretics. The preliminary prognostication of ligand-target interactions and affinity levels allow to reduce quantity of experimental animals, synthesis, and pharmacological studies costs. Conducted studies revealed the series of promising 6,7-disubstituted pteridine-2,4(1H,3H)-diones with diuretic activity that comparable with pharmacological effect of triamterene. Aim – purposeful search for promising diuretics among structural analogues of triamterene that includes preliminary in silico studies, synthesis and in vivo screening of novel compounds for diuretic activity. Methods used: organic synthesis, physicochemical methods of analysis of organic compounds (NMR 1H-spectroscopy, chromato-mass spectrometry, elemental analysis). Prediction of affinity for a biological target, prediction of toxicity and lipophilicity of the combinatorial library, which was created on the basis of the drug triamterene, was carried out using computer services. Studies of compounds that affect the excretory function of the kidneys of rats were performed according to the generally accepted method of E.B. Berkhin with water load. Research of the probable mechanism was conducted by flexible molecular docking, as an approach of finding molecules with affinity to a specific biological target. Macromolecular data were downloaded from the Protein Data Bank (PDB) namely, the crystal structures of epithelial sodium channel (ENaC) ((PDB ID – 6WTH). The substantiation of potential diuretics design was conducted by in silico methods (prediction of affinity, ligand-enzyme interactions and pharmacokinetic characteristics). The structural modification of triamterene molecule was carried out by replacing of amino-group in positions 2, 4 and 7 by others “pharmacophore” fragments. Abovementioned transformation is aimed at the changing of ligand-enzyme interactions in active site, lipophility and toxicity. Synthesis of 6,7-disubstituted pteridine-2,4(1H,3H)-diones was conducted by condensation 5,6-diamino-2-oxo-(thioxo-)-2,3-dihydropyrimidin-4(1H)-ones with carbonyl-containing compounds or oxocarboxylic acids. The further modification of obtained compounds was performed by alkylation, hydrazinolysis and nucleophilic addition/elimination. The structure of obtained compounds was proven by elemental analysis, chromato-mass and 1H NMR-spectral analysis. The studies of synthesized compounds effect on excretion function of kidneys allowed to detect series of promising structural analogues of triamterene that exceed it in pharmacological activity by 27.3-99.0%. The “structure-biological activity” relationship was discussed and perspective of the further search of diuretics among abovementioned compounds were shown. The design of new biologically active compounds with diuretic activity was performed using in silico methodologies and realized by structural modification of the well-known diuretic triamterene. Traditional organic synthesis was used for preparation of target compounds, in vivo experiments were used to detect compounds with significant biological activity. Several effective compounds were identified among pteridines, which exceed the reference drug triamterene in terms of daily diuresis. The obtained results substantiate further purposeful search, in-depth research on experimental pathologies and study of the mechanism of action of potential diuretics among this class of compounds.
- Paul D, SanapG, Shenoy S, Kalyane D, Kiran K, Tekade Artificial intelligence in drug discovery and development. Drug Discov Today. 2021;26(1):80-93. doi: https://doi.org/10.1016/j.drudis.2020.10.010
- Epithelial sodium channels (ENaC). British Journal of Pharmacology. 2009;158:S137-8. doi: https://doi.org/10.1111/j.1476-5381.2009.00503_9.x
- Kellenberger S, Schild L. International Union of Basic and Clinical Pharmacology. XCI. Structure, Function, and Pharmacology of Acid-Sensing Ion Channels and the Epithelial Na+ Channel. Pharmacological Reviews. 2015;67:1-35. doi: http://dx.doi.org/10.1124/pr.114.009225
- Carmona-Martínez V, Ruiz-Alcaraz AJ, Vera M, Guirado A, Martínez-Esparza M, García-Peñarrubia P. Therapeutic potential of pteridine derivatives: A comprehensive review. Med Res Rev. 2018;1-56. doi: https://doi.org/10.1002/med.21529
- Roush GC, Sica DA. Diuretics for Hypertension: A Review and Update. American Journal of Hypertension. 2016;29(10):1130-7. doi: https://doi.org/10.1093/ajh/hpw030
- Rossiter S, Ostovar M. Bicyclic 6-6 Systems: Pteridines, Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. Comprehensive Heterocyclic Chemistry IV. 2022;10:796-855. doi: https://doi.org/10.1016/B978-0-12-818655-8.00040-8
- Sokolova KV, Stavytskyi VV, Kovalenko SI, Podpletnya OA. Directed search for diuretics among 6-substituted pteridine-2,4,7(1H,3H,8H)-triones. Medicni perspektivi. 2022;2:4-15. doi: https://doi.org/10.26641/2307-0404.2022.2.260051
- Protein Data Bank. [Internet]; [сited 2022 May 11]. Available from: http://www.rcsb.org/pdb/home/home.-do
- MarvinSketch version 20.20.0, ChemAxon. [Internet]. Available from: http://www.chemaxon.com
- Trott O, Olson AJ. AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem. 2010;31(2):455-61. doi: https://doi.org/10.1002/jcc.21334
- Discovery Studio Visualizer v19.1.018287. Accelrys Software Inc., [Internet]. [сited 2022 May 11]. Available from: https://www.3dsbiovia.com.
- ProTox-II – Prediction of toxicity of chemicals. [Internet]. [сited 2022 May 11]. Available from: https://tox-new.charite.de/protox_II/index.php?site=home.-do
- European convention for the protection of vertebrate animal used for experimental and other scientific purposes. Council of Europe, Strasbourg; 1986.
- Briukhanov VM, Zverev YuF, Lampatov VV, Zharikov AYu. [Methodological approaches to the study of kidney function in animal experiments]. Nefrolohiia. 2009;13(3):52-62.
- Stefanov OV. [Preclinical studies of drugs]. Kyiv: Avitsena; 2001.
- Lapach SN, Chubenko AV, Babich PN. [Statistical methods in biomedical research using EXCEL]. Kyiv: Morion; 2000.
- Kazunin M, Voskoboynik O, Nosulenko I, Berest G, Sergeieva T, Okovytyi S, et al. Synthesis, tautomerism and antiradical activity of 1-methyl-6-(2-(aryl-(hetaryl-))-2-oxoethyl)pteridine-2,4,7(1H, 3H, 6H)-triones. Heterocyclic Chem. 2018;4:1033-41. doi: https://doi.org/10.1002/jhet.3135
- Kazunin M, Voskoboynik O, Nosulenko I, Berest G, Kholodnyak S, Pryшmenko B, Kovalenko S. Synthesis, antiradical and antimicrobial activity of new pteridine-2,4,7-trione derivatives. J. Heterocyclic Chem. 2019;1-13. doi: https://doi.org/10.1002/jhet.3774
- Breitmaier E. Structure Elucidation by NMR in Organic Chemistry: A Practical Guide’, Eberhard Breitmaier, 3rdRevised Edition. Wiley; 2002. doi: https://doi.org/10.1002/0470853069
- Carruthers W, Coldham I. Modern Methods of Organic Synthesis 4th edition, Cambridge University Press. 2004. doi: https://doi.org/10.1017/CBO9780511811494
Popko S.S. Dynamics of the cellular composition of lymphoid nodules in the lungs of guinea pigs sensitized with ovalbumin
Key words: lymphoid nodule, guinea pig, lung, allergic inflammation, ovalbumin
Abstract. Dynamics of the cellular composition of lymphoid nodules in the lungs of guinea pigs sensitized with ovalbumin. Popko S.S. The article discusses the morphological aspects of the dynamics of the cellular composition of lymphoid nodules in the lungs of guinea pigs as a result of an experimental ovalbumin-induced allergic process. We studied the reactivity of immunocompetent cells of lymphoid formations of the lungs after three times subcutaneous sensitization and subsequent 8-day intranasal aeroallergization with ovalbumin in the early and late stage period of the allergic inflammatory process by microscopic, morphometric and statistical methods. By help of morphometric analysis we demonstrate the general regularity of reactivity of a local specific link of the pulmonary immune system to the action of an allergen, which consists in the elevation of the average amount of immune cells of lymphoid nodules of the lungs, starting from the 30th to the 44th day after the start of the experiment. The maximal coefficient of increase by 5.8 times was observed in counting plasma cells among all types of immunocompetent cells of lymphoid nodules in the lungs during the experiment. It has been statistically proven that the implementation of the ovalbumin-induced allergic inflammatory process in the lungs proceeds according to the humoral type and the duration of its course is not limited by the direct influence of the allergen, it also continues after the end of its action, which is a manifestation of changes in compensatory-adaptive processes in the pulmonary immune system with ovalbumin-induced allergic inflammation.
- Dey P. Basic and Advanced Laboratory Techniques in Histopathology and Cytology. Singapore: Springer; doi: https://www.springer.com/gp/book/9789811082511
- Hwang JY, Randall TD, Silva-Sanchez A. Inducible Bronchus-Associated Lymphoid Tissue: Taming Inflammation in the Lung. Frontiers in Immunology. 2016 Jun 30;7(258). doi: https://doi.org/10.3389/fimmu.2016.00258
- Moro K, Kabata H, Tanabe M, Koga S, Takeno N, Mochizuki M, et al. Interferon and IL-27 antagonize the function of group 2 innate lymphoid cells and type 2 innate immune responses. Nature Immunology. 2015 Nov 23;17(1):76-86. doi: https://doi.org/10.1038/ni.3309
- Lambrecht BN, Hammad H. The immunology of asthma. Nature Immunology. 2014 Dec 18;16(1):45-56. doi: https://doi.org/10.1038/ni.3049
- Petrie A, Sabin C. Medical Statistics at a Glance. 4th ed. Wiley-Blackwell; 2019.
- Popko SS, Evtushenko VM, Syrtsov VK. Influence of pulmonary neuroendocrine cells on lung homeostasis. Zaporozhye medical journal. 2020;22(4):568-75. doi: https://doi.org/10.14739/2310-1210.2020.4.208411
- Baluk P, Adams A, Phillips K, Feng J, Hong Y-K, Brown MB, et al. Preferential Lymphatic Growth in Bronchus-Associated Lymphoid Tissue in Sustained Lung Inflammation. The American Journal of Pathology. 2014 May;184(5):1577-92. doi: https://doi.org/10.1016/j.ajpath.2014.01.021
- Klose CSN, Mahlakõiv T, Moeller JB, Rankin LC, Flamar A-L, Kabata H, et al. The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation. 2017;549(7671):282-6. doi: https://doi.org/10.1038/nature23676
- Bahriy MM, Dibrova VA, Popadynets' OH, Hryshchuk MI, Bahriy MM. [Methods of morphological research: monograph]. Vinnytsia: Nova knyha; 2016. Ukrainian.
- Plas DzhV. [Python for complex tasks. Data Science and Machine Learning]. SPb.: Pyter; 2018. Russian.
Bychkova S.V., Stasyshyn A.R., Bychkov M.A. The role of bafilomycin as a therapeutic agent in the modulation of endo-lysosomal store of rat hepatocytes
Authors and affiliations
Stasyshyn A.R. Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Bychkov M.A. Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Key words: аutophаgу, hepatocytes, bаfіlomycіn A1, ATPases, NAAD, Ca2+-store
Abstract. The role of bafilomycin as a therapeutic agent in the modulation of endo-lysosomal store of rat hepatocytes. Bychkova S.V., Stasyshyn A.R., Bychkov M.A. Endo-lysosomal system through the process of autophagy is involved in the pathogenesis of many diseases. Acidification of these organelles is carried out by V-type H+-ATPases, which is inhibited by bafilomycin A1. Endosomes and lysosomes are also important Ca2+-storage in a cell. Nіcotіnіc acіd adenіne dіnucleotіde phosphate (NAADP) releases Cа2+ from endo-lysosomes. The main purpose of the study was to found out the effect of bafilomycin A1 and NAADP on stored Ca2+ and on the ATPase activity of rat hepatocytes. The stored Ca2+ was estimated using chlorotetracycline in permeabilized hepatocytes of rats. ATPase activity was determined by level of orthophosphate spectrophotometrically. It was found that bafilomycin A1 reduces stored Ca2+ in permeabilized hepatocytes of rats in the micromolar range of concentration (20 and 0.04 mkM) and averted the effect of NAADP on calcium content. Lower concentrations of bafilomycin A1 (0.001 mkM) did not alter the content of stored calcium, but prevented the influence of NAADP in permeabilized hepatocytes of rats. In the subcellular fraction of rat liver bafilomycin A1 (0.001 mkM) increased Ca2+-ATPase and basal Mg2+-ATPase activities and reduced Na+/K+-ATPase activity. Preincubation of the subcellular fraction with bafilomycin A1 completely averts any changes in the activity of estimated ATPases by means of NAADP. It was concluded that the bafilomycin-sensitive store in hepatocytes of rats is NAADP-sensitive endo-lysosomal Ca2+-store. Using of bafilomycin A1 may be useful in treating autophagy-depended diseases.
- Bychkova S. Influence of NAADP and bafilomycine A1 on activity of ATPase in liver postmitochondrial fraction. Studia Biologica. 2015;9(3-4):31-40. doi: https://doi.org/10.30970/sbi.0903.445
- Bychkova S, Chorna T. NAADP-sensitive Сa2+ stores in permeabilized rat hepatocytes. Ukr. Biochem. J. 2014;86(5):65-73. doi: https://doi.org/10.15407/ubj86.05.065
- Bychkova S, Hreniuh V. Activity of ATPases in postmitoсhondrial fraction of lymphoma NK/Ly cells under bafilomicine and NAADP presence mitochondria. Studia 2015;9(2):31-38. doi: https://doi.org/10.30970/sbi.0902.413
- Fay MP, Proschan Wilcoxon-Mann-Whitney or t-test? On assumptions for hypothesis tests and multiple interpretations of decision rules. Stat Surv. 2010;4:1-39. doi: https://doi.org/10.1214/09-SS051
- Ferents IM, Bychkova SV, Bychkov MA. Peculiarities of the effects of bile acids on atpase activity of the colon mucosa in patients with overweight and irritable bowel syndrome. Wiadomosci lekarskie. 2020;73(3):574-7. doi: https://doi.org/10.36740/WLek202003133
- Hreniukh V, Bychkova S, Kulachkovsky O, Babsky A. Effect of bafilomycin and NAADP on membrane associated ATPases and respiration of isolated mitochondria of the murine Nemeth-Kellner lymphoma. Cell biochemistryand 2016;34(8):579-87. Epub 2016 Nov 8. doi: https://doi.org/10.1002/cbf.3231
- Mauvezin C, Neufeld TP. Bafilomycin A1 disruptsautophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome Autophagy. 2015;11(8):1437-8. doi: https://doi.org/10.1080/15548627.2015.1066957
- Mindell, Joseph A. Lysosomal Acidification Mechanisms. Annual Review of Physiology. 2012;74:69-86. doi: https://doi.org/10.1146/annurev-physiol-012110-142317
- Zhang B, Watt JM, Cordiglieri C, et al. Small Molecule antagonists of NAADP-induced Ca2+ release in T-Lymphocytes suggest potential therapeutic agents for autoimmune disease. Nature: Scientific reports: 2018;8:16775. doi: https://www.nature.com/articles/s41598-018-34917-3
Kozlova Yu.V., Maslak H.S., Abraimova O.E., Koldunov V.V., Khudyakov O.E. State of spatial memory and antioxidant system activity of rats in the dynamics of development of blast-induced traumatic brain injury
Authors and affiliations
Maslak H.S. Dnipro State Medical University, Dnipro, Ukraine
Abraimova O.E. Dnipro State Medical University, Dnipro, Ukraine
Koldunov V.V. Dnipro State Medical University, Dnipro, Ukraine
Khudyakov O.E. Dnipro State Medical University, Dnipro, Ukraine
Key words: blast-induced traumatic brain injury, brain, spatial memory, catalase
Abstract. State of spatial memory and antioxidant system activity of rats in the dynamics of development of blast-induced traumatic brain injury. Kozlova Yu.V., Maslak H.S., Abraimova O.E., Koldunov V.V., Khudyakov O.E. The main purpose of this study was to investigate the changes in spatial memory and catalase activity in dynamics in the blast-induced traumatic brain injury (bTBI). The experiment was carried out on 67 albino male Wistar rats, which were randomly divided into three groups: I group – experimental (n=34), animals were subjected to inhalation anesthesia with halothane, fixed and was simulated blast-induced traumatic brain injury was simulated by generating a shock wave with an overpressure of 26.4±3.6 kPa, II group – sham (n=34), animals which were subjected only to inhalation anesthesia and fixation and III group – intact (n=34). After the study of behavior, euthanasia was performed, blood and brain were taken shelter. Histopathological examination of the brain showed disturbances both of hippocampal neurons and microcirculature. In the dynamics of the post-traumatic period of mild bTBI there was observed a significant (p<0.01) impairment of rats spatial memory in the experimental group, which was established as a prolongation of latent time of the search in the Barnes maze: by 39% – on day 1, by 76% – on day 3, by 65% – on day 7 and by 61% – on day 14 of the study. The analysis of catalase activity revealed a significant decrease of this enzyme activity in blood plasma of the rats in the experimental group in comparison with rats of sham and intact groups: on day 1 – by 35% (p<0.01), on day 3 – by 27% (p<0.01) and by 12% on day 7 of the post-traumatic period (p<0.05), which indicates the involvement of catalase in the reaction of hydrogen peroxide inactivation. Correlation analysis between spatial memory and catalase activity in experimental rats showed a negative relationship of medium degree on day 1 (r=-0.3, p<0.01) and a negative relationship (r=-0.2, p<0.01) of weak degree on day 3 of post-traumatic period, indicating increased formation of free radicals during this period. One-way analysis of variance showed a significant (H1, p<0.01) effect of changes in the catalase activity of rat blood plasma on the state of spatial memory in the Barnes maze during 14 days of the post-traumatic period. It was established that oxidative stress is an important link in pathogenesis of the spatial memory disorders in animals with mild bTBI in the first week of the post-traumatic period.
- Antamonov MYu. [Mathematical processing and analysis of medico-biological data]. 2nd ed. Kyiv: MIC “Medinform”; 2018. Russian.
- Ershova IV. [Erythrocyte catalase activity in forensic medical diagnosis of recurrent craniocerebral trauma and trauma sustained while intoxicated, abstract]. Moskva: GOU VPO "Pervyi Moskovskii gosudarstvennyi universitet im. I.M. Sechenova"; 2011. Russian.
- Kozlov SV, Mishalov VD, Suloiev KM, Kozlova YuV. [Pathomorphological markers of blast-induced brain injury]. Morfologiia. 2021;15(3):96-100. Ukrainian. doi: https://doi.org/10.26641/1997-9665.2021.3.96-100
- Korzhevskii DJe, Gilerovich EG, Kirik OV. [Morphological diagnostics: preparation of material for histological examination and electron microscopy: a practical guide]. Sankt-Peterburg: SpecLit; 2013. Russian.
- Kozhemyakin YuM, Khromov OS, Boldyreva NE, Dobrelya V, Saifetdinova GA, editors. [Scientific and practical recommendations for keeping and working with laboratory animals: manual]. Kyiv: Interservice; 2017. ISBN 978-617-696-560-2. Ukrainian.
- Kozlova YuV, Abdul-Ogly LV, Kosharnyi AV, Kytova IV, Korzachenko MA, inventor; [Device for studying the effect of the shock wave of an explosion on the body]. Ukraine patent N 146858. 2021. Ukrainian. Available from: https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=275098
- Phipps H, Mondello S, Wilson A, Dittmer T, Rohde NN, Schroeder PJ, et al. Characteristics and Impact of U.S. military blast-related mild traumatic brain injury: a systematic review. Front Neurol. 2020;11:559318. doi: https://doi.org/10.3389/fneur.2020.559318
- Cheryl SR, Sherry AF. Barnes maze testing strategies with small and large rodent models. J. Vis. Exp. 2014;84:1-15. doi: https://doi.org/10.3791/51194
- Cobley JN, Fiorello ML, Bailey DM. 13 reasons why the brain is susceptible to oxidative stress. Redox Biol. 2018;15:490-503. doi: https://doi.org/10.1016/j.redox.2018.01.008
- Rodriguez UA, Zeng Y, Deyo D, Parsley MA, Hawkins BE, Prough DS, DeWitt DS. Effects of mild blast traumatic brain injury on cerebral vascular, histopathological, and behavioral outcomes in rats. J Neurotrauma. 2018;35(2):375-92. doi: https://doi.org/10.1089/neu.2017.5256
- Ho MH, Yen CH, Hsieh TH, Kao TJ, Chiu JY, Chiang YH, et al. CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury.Redox 2021;46:102067. doi: https://doi.org/10.1016/j.redox.2021.102067
- Honig MG, Dorian CC, Worthen JD, Micetich AC, Mulder IA, Sanchez KB, et al. Progressive long-term spatial memory loss following repeat concussive and subconcussive brain injury in mice, associated with dorsal hippocampal neuron loss, microglial phenotype shift, and vascular abnormalities. Eur J Neurosci. 2021;54(5):5844-79. doi: https://doi.org/10.1111/ejn.14711
- Pylypenko SV, Koval AA, Akarchuk V. Activity of superoxide dismutase and catalase in the gastric mucosa of rats under the prolonged administration of omeprazol and combination of omeprazole and multiprobiotics. Wiadomosci 2021;74(2), 317-20. doi: https://doi.org/10.36740/WLEK202102127
- Kuriakose M, Younger D, Ravula AR, Alay E, Rama Rao KV, Chandra N. Synergistic role of oxidative stress and blood-brain barrier permeability as injury mechanisms in the acute pathophysiology of blast-induced neurotrauma.Sci 2019;9(1):7717. doi: https://doi.org/10.1038/s41598-019-44147-w
- Whitney K, Nikulina E, Rahman SN, Alexis A, Bergold PJ. Delayed dosing of minocycline plus N-acetylcysteine reduces neurodegeneration in distal brain regions and restores spatial memory after experimental traumatic brain injury. Experimental neurology. 2021;345:113816. doi: https://doi.org/10.1016/j.expneurol.2021.113816
Degtiar A.V. Morphology of the rat knee joint after intraarticular injection of hydroxymethyl-quinoxaline dioxide
Key words: hydroxymethyl-quinoxaline dioxide, experiment, rats, knee joint, histology
Abstract. Morphology of the rat knee joint after intraarticular injection of hydroxymethyl-quinoxaline dioxide. Degtiar A.V. Topical antiseptics are widely used in orthopedics and traumatology. However, studies highlighting the effects of antiseptics during long-term use on joint tissues are limited. In the performed work, the peculiarities of the structure of intra-articular tissues of the knee joint and muscles in rats in two series of experiments were noted: control group (saline injection into the joint) and experimental group (hydroxymethyl-quinoxaline dioxide injection into the joint) daily for 5 days. Histological methods with semi-quantitative assessment of articular cartilage and synovial membrane were used. During the first day after the drug administration, similar reactive changes to the injection of saline and hydroxymethyl-quinoxaline dioxide were observed in the joint tissues of the two groups of rats. The synovial membrane of the capsule was thickened, and synoviocytes were hypertrophied. The articular cartilage contains single cells with dense nuclei only in the superficial parts, but in general, the cytoarchitectonics of articular cartilage was preserved. In 5 days after injection, no statistically significant differences between saline and hydroxymethyl-quinoxaline dioxide were observed for the study periods. According to the studies the safety of long-term use of the drug as an antiseptic for articular tissues was confirmed.
- Fuchs M, Pumberger M, Hommel H, Perka C, von Roth P, Thiele K. Bacterial Colonization of Irrigation Fluid during Aseptic Revision Knee Arthroplasty. J Clin Med. 2020;9(9):2746. doi: https://doi.org/10.3390/jcm9092746
- Loskutov AE. Dehtyar AV, Ctepanskyi DA. [Comparative evaluation of the antimicrobial activity of local antiseptics in revision hip arthroplasty]. Medicni perspektivi.2017;22(1):25-32. doi: https://doi.org/10.26641/2307-0404.2017.1.100870
- Shahi A, Parvizi J. Prevention of Periprosthetic Joint Infection. Arch Bone Jt Surg. 2015;3(2):72-81. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468618/
- Calkins TE, Culvern C, Nam D, Gerlinger TL, Levine BR, Sporer SM et al. Dilute betadine lavage reduces the risk of acute postoperative periprosthetic joint infection in aseptic revision total knee and hip arthroplasty: a randomized controlled trial. J Arthroplasty. 2019;35(2):538-43. doi: https://doi.org/10.1016/j.arth.2019.09.011
- Goswami K, Austin MS. Intraoperative povidone-iodine irrigation for infection prevention. Arthroplast Today.2019;5:306-8. doi: https://doi.org/10.1016/j.artd.2019.04.004
- Koburger T, Hubner NO, Braun M, Siebert J, Koburger T. Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, poly-hexanide and chlorhexidine digluconate. Antimicrob Chemother.2010;5(8):1712-9. doi: https://doi.org/10.1093/jac/dkq212
- Wood T, Ekhtiari S, Mundi R, Citak M, Sancheti PK, Guerra-Farfan E, et al. The Effect of Irrigation Fluid on Periprosthetic Joint Infection in Total Hip and Knee Arthroplasty: A Systematic Review and Meta-Analysis. 2020;12(4):e7813. doi: https://doi.org/10.7759/cureus.7813
- Akhtyamov IF, Rimashevskii DV, Kurmangaliev Е-DT, Moldakulov ZhM, Eremin IK. [Prevention of infectious complications of knee arthroplasty]. Meditsinskaia praktika. Kazan, 2014. Russian.
- Hart A, Hernandez NM, Abdel MP, Mabry TM, Hanssen AD, Perry KI. Povidone-iodine wound lavage to prevent infection after revision total hip and knee arthroplasty. J Bone Joint Surg. 2019;101(13):1151-59. doi: https://doi.org/10.2106/JBJS.18.01152
- European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasburg; 1986(123). Available from: https://rm.coe. int/168007a67b
- [Order of the Ministry of Education and Science of Ukraine No. 249 of March 1, 2012 “Procedure for conducting experiments and experiments on animals by scientific institutions].(2012). Available from: https://zakononline.com.ua/documents/show/218506___218571
- Sarkisov DS, Perov YuL, editors. [Microscopic technique. A guide for physicians and laboratory assistants]. Moskva: Meditsina; 1996. Russian.
- GerwinN, Bendele AM, Glasson S, Carlson The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rat. 2010;18(Suppl. 3):S24-34. doi: https://doi.org/10.1016/j.joca.2010.05.030
- Grzhibovsky AM, Ivanov SV, Gorbatova MA. [Comparison of Quantitative Data from Two Independent Samples Using Statistica and SPSS Software: Parametric and Nonparametric Tests]. Nauka i zdravoohranenie. 2016;2:5-28. Available from: https://cyberleninka.ru/article/n/sravnenie-kolichestvennyh-dannyh-dvuh-nezavisimyh-vyborok-s-ispolzovaniem-programmnogo-obespecheniya-statistica-i-spss
- Boddapati V, Fu MC, Mayman DJ, Su EP, Sculco PK, McLawhorn AS. Revision total knee arthroplasty for periprosthetic joint infection is associated with increased postoperative morbidity and mortality relative to noninfectious revisions. J Arthroplast. 2018;33:521-6. doi: https://doi.org/10.1016/j.arth.2017.09.021
- `Wood T, Ekhtiari S, Mundi R, Citak M, Sancheti PK, Guerra-Farfan E, et al. The Effect of Irrigation Fluid on Periprosthetic Joint Infection in Total Hip and Knee Arthroplasty: A Systematic Review and Meta-Analysis. 2020;12(4):e7813. doi: https://doi.org/10.7759/cureus.7813
- Frisch NB, Kadri OM, Tenbrunsel T, Abdul-Hak A, Qatu M, Davis JJ. Intraoperative chlorhexidine irrigation to prevent infection in total hip and knee arthroplasty. Arthroplast Today. 2017;12:294-97. doi: https://doi.org/10.1016/j.artd.2017.03.005
- Dehtyar VA, Dehtyar AV. Dedukh NV, Nikolchenko OA. [Short and long-term effects of the antiseptic decametoxin (dekasan®) on structural components of the knee joint in conditions intra-articular introduction (experimental study)]. Visnyk problem biolohii i medytsyny. 2019;1-2(149):286-90. Ukrainian. doi: https://doi.org/10.29254/2077-4214-2019-1-2-149-286-290
Shulyatnikova T.V., Tumanskiy V.O. Brain aquaporin-4 expression in the rat septic model (immunohistochemical study)
Key words: sepsis-associated encephalopathy, astroglial reactivity, aquaporin-4
Abstract. Brain aquaporin-4 expression in the rat septic model (immunohistochemical study). Shulyatnikova T.V., Tumanskiy V.O. The study aimed to determine aquaporin-4 expression in different brain regions was performed in Wistar rats subjected to cecal ligation and puncture (CLP) septic model. The immunohistochemical study of aquaporin-4 was carried out in the sensorimotor cortex, white matter, hippocampus, thalamus and caudate nucleus/putamen regions between 20 and 38 h after CLP. From the 12th h after CLP all animals showed the progressive impairment of sepsis signs and therefore, 9 rats were euthanized between 20-38 h (“CLP-B”, non-survived); 11 animals survived up to 48 h (constituted “CLP-A”, survived). After operation, CLP-B group displayed regionally-specific dynamic increase in aquaporin-4 level in the brain mostly associated with astroglial capillary endfeet: by 23rd h in the cortex – 234.15%, by 24th h in the thalamus –129.47% and hippocampus – 101.36%, by 30th h in the white matter – 135.31% and by 38 h in the caudate/putamen – 92.85%; with the highest increase in cortex: by 3.34 times. Heterogeneous and heterochronous aquaporin-4 elevation among brain regions indicates territories more and less susceptible for systemic toxic exposure in sepsis as well as points to diverse reactive responsiveness of local astroglial populations during specific time-period after CLP. The higher rates of aquaporin-4 in the cortex of non-survived animals in CLP model reflects the importance of aquaporin-4 increase in the mechanisms of sepsis decompensation.
- Mazeraud A, Righy C, Bouchereau E, Benghanem S, Bozza FA, Sharshar T. Septic-Associated Encephalopathy: a Comprehensive Review. Neurotherapeutics. 2020 Apr;17(2):392-403. doi: https://doi.org/10.1007/s13311-020-00862-1
- Chung HY, Wickel J, Brunkhorst FM, Geis C. Sepsis-Associated Encephalopathy: From Delirium to Dementia? JClin 2020 Mar 5;9(3):703. doi: https://doi.org/10.3390/jcm9030703
- Rump K, Adamzik M. Function of aquaporins in sepsis: a systematic review. Cell Biosci. 2018 Feb 9;8:10. doi: https://doi.org/10.1186/s13578-018-0211-9
- Ramadhanti J, Yamada T, Yasui M, Nuriya M. Differentially regulated pools of aquaporin-4 (AQP4) proteins in the cerebral cortex revealed by biochemical fractionation analyses. J Pharmacol Sci. 2021 May;146(1):58-64. doi: https://doi.org/10.1016/j.jphs.2021.03.003
- Vandebroek A, Yasui M. Regulation of AQP4 in the Central Nervous System. Int J Mol Sci. 2020 Feb 26;21(5):1603. doi: https://doi.org/10.3390/ijms21051603
- Tradtrantip L, Jin BJ, Yao X, Anderson MO, Verkman AS. Aquaporin-Targeted Therapeutics: State-of-the-Field. Adv Exp Med Biol. 2017;969:239-50. doi: https://doi.org/10.1007/978-94-024-1057-0_16
- Verkman AS, Smith AJ, Phuan PW, Tradtrantip L, Anderson MO. The aquaporin-4 water channel as a potential drug target in neurological disorders. Expert Opin Ther Targets. 2017 Dec;21(12):1161-70. doi: https://doi.org/10.1080/14728222.2017.1398236
- Gong W, Wen H. Sepsis Induced by Cecal Ligation and Puncture. Methods Mol Biol. 2019;1960:249-55. doi: https://doi.org/10.1007/978-1-4939-9167-9_22
- Shulyatnikova T, Shavrin V. Mobilisation and redistribution of multivesicular bodies to the endfeet of reactive astrocytes in acute endogenous toxic encephalopathies. Brain Res. 2021 Jan 15;1751:147174. doi: https://doi.org/10.1016/j.brainres.2020.147174
- Kim SW, Roh J, Park CS. Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips. J Pathol Transl Med. 2016 Nov;50(6):411-8. doi: https://doi.org/10.4132/jptm.2016.08.08
- Antomonov MYu. [Mathematical processing and analysis of medical and biological data. 2nd ed.]. Кyiv: MYCz "Medynform"; 2018. р. 579. Russian.
- Sfera A, Price AI, Gradini R, Cummings M, Osorio C. Proteomic and epigenomic markers of sepsis-induced delirium (SID). Front Mol Biosci. 2015 Oct 26;2:59. doi: https://doi.org/10.3389/fmolb.2015.00059
- Filippidis AS, Carozza RB, Rekate HL. Aquaporins in Brain Edema and Neuropathological Conditions. Int J Mol Sci. 2016 Dec 28;18(1):55. doi: https://doi.org/10.3390/ijms18010055
- Meli R, Pirozzi C, Pelagalli A. New Perspectives on the Potential Role of Aquaporins (AQPs) in the Physiology of Inflammation. Front Physiol. 2018 Feb 16;9:101. doi: https://doi.org/10.3389/fphys.2018.00101
- Ikeshima-Kataoka H. Neuroimmunological Implications of AQP4 in Astrocytes. Int J Mol Sci. 2016 Aug 10;17(8):1306. doi: https://doi.org/10.3390/ijms17081306
- Shulyatnikova T, Tumanskiy V. Glutamine synthetase expression in the brain during experimental acute liver failure (immunohistochemical study). J Educ Health Sport. 2021 Oct;11(10):342-56. doi: https://doi.org/10.12775/jehs.2021.11.10.033
Astakhov V.M., Batsylyeva O.V., Puz I.V., Shudrikova N.V. Features of the organization of medical and psychological assistance in the situation of perinatal losses (literature review)
Authors and affiliations
Key words: perinatal loss, grief reaction, psycho-emotional state, pregnancy, medical and psychological assistance
Abstract. Features of the organization of medical and psychological assistance in the situation of perinatal losses (literature review). Astakhov V.M., Batsylyeva O.V., Puz I.V., Shudrikova N.V. The article reveals the organizational features of medical and psychological assistance in cases of perinatal losses in modern conditions. The paper presents the peculiarities of perinatal loss, psychological condition of women’s health during the onset of subsequent pregnancy as well as experiences of the women who have lost a child at different stages of pregnancy. The article notes that the majority of pregnant women with a history of perinatal loss are in a state of chronic stress, which can lead to the emergence and development of various medical and psychological complications. The peculiarities of the experience of grief in the situation of perinatal loss, as a serious mental trauma for the woman's psyche were analyzed. It has been substantiated that the experience of perinatal loss is a non-normative crisis in marital relations, and the process of providing psychological assistance should be directed not only to the woman, but also to the married couple as a whole, which will reduce the risk of rupture of relations, promote their harmonization, which can be considered as an effective resource for a constructive experience of the situation of loss. The main tasks of psychologists and medical staff during the organization of medical and psychological assistance for women and married couples experiencing perinatal loss are also revealed. It is crucial to comprehend the feelings women experience in such cases, which makes medical and psychological care more effective in the process of working with those who are through child loss or have had such an experience previously. The conclusion notes the priority of organizing and providing comprehensive medical and psychological assistance not only to women, but also to married couples in a situation of perinatal loss; the need for special training both for medical psychologists, obstetricians and gynecologists to provide complex assistance to women experiencing perinatal losses.
- Astakhov VM, Batsylyeva OV, Puz IV. [Psychological accompaniment of pregnancy in the modern condition]. Zdorove zhenshchiny. 2014;4(90):58-61. Russian. Available from: http://nbuv.gov.ua/UJRN/Zdzh_2014_4_12
- Bala OO, Benyuk VA, Kovalyuk TV, Benyuk SV. [Features of psychoemotional state of pregnant women with missed abortion in the early stages of pregnancy]. Perinatolohiia i pediatriia. 2016;2(66):40-42. Ukrainian doi: https://doi.org/10.15574/PP.2016.66.40
- Baranova VV. [Psychoemotional peculiarities of women who have a history of miscarriage and a loss during the current pregnancy]. Perynatolohiia i pediatriia. 2017;1(69):70-73. Ukrainian. doi: https://doi.org/10.15574/PP.2017.69.70
- [The program of psychological support for pregnant women with perinatal losses in anamnesis]. In: Batsylyeva OV, Astakhov VM, Puz IV, editors. Kharkiv autumn marathon of psychotechnologies (catalogue of psychotechnologies; theses of reports). Materials of the 3rd interregional science and practice conference; 2019 Oct. 26; Kharkiv, Ukraine. Kharkiv: Disa plius; 2019. p. 15-17. Ukrainian.
- Bulavenko OV, Rud VO, Shinkaruk LV. [Miscarriage in women with a history of stillborn pregnancy]. Zdorove zhenshchiny. 2014;9:52-56. Ukrainian. Available from: http://nbuv.gov.ua/UJRN/Zdzh_2014_9_9
- Vovk IB, Vdovychenko YuP, Trokhymovych OV, Zadorozhna TD, Horovenko NH. [Early reproductive losses]. Kyiv; 2016. Ukrainian.
- Vorden V. [Counseling and grief therapy: a guide for mental health professionals]. Moskva: Centr psihologicheskogo konsultirovaniya i psihoterapii; 2020. Russian.
- Vorobyova II, Zhivetskaya-Denisova AA, Tkachenko VB, Rudakova NV, Tolkach SM. [Miscarriage of pregnancy: current views on the problem (literature review)]. Zdorove zhenshchiny. 2017;3(119):113-7. Ukrainian. Available from:
- Grabarovska L, Solovova N. [Corrective work with patients who have experienced perinatal loss]. European Research. 2015;10:138-41. Russian. Available from: https://elibrary.ru/item.asp?id=25186908
- Dobryakov IV, Blokh ME, Faerberg LM [Perinatal loss: psychological aspects, the specificity of mourning, forms of psychological help]. Zhurnal akusherstva i zhenskih boleznei. 2015;3:4-10. Russian. doi: https://doi.org/10.17816/JOWD6434-10
- Zhuk SI, Shchurevska OD, Shlyahtina AA. [Perinatal loss – psychological aspects of the obstetricians and gynecologists work (clinical lecture)]. Zdorove zhenshchiny. 2017;1(117):1-13. Ukrainian. Available from: https://med-expert.com.ua/journals/wpcontent/uploads/2017/06/WH_01_ 2017_web-2.pdf
- Zlakotina NA. [Psychological support of perinatal losses]. Aktualnye problemy humanitarnykh i estestvennykh nauk. 2015;2:142-6. Russian. Available from: https://publikacia.net/archive/2015/8/2/44
- Kaminskiy V, Ventskovskaia I, Zhdanovych O, Vorobey L, Kolomiichenko T. [Peculiarities of Psychoemotional Status of Pregnant Women with Perinatal Losses in the History]. Psihiatriia, psihoterapiia i klinicheskaia psihologiia. 2020;1(11):66-74. Russian. doi: https://doi.org/10.34883/PI.2020.11.1.006
- Kaminskiy VV, Zhdanovych OI, Vorobey LI, Kolomiichenko TV, Rogava IV. [Perinatal losses in anamnesis as a factor of fetus adaptation damage]. Reproduktyvna endokrynolohiia. 2019;3(47):48-52. Ukrainian.
- Mihailov AV, Zamanaeva YuV. [Psychology perinatal loss: do no harm]. Status Praesens. 2015;6:104-12. Russian. Available from: https://www.elibrary.ru/contents.asp?id=34482656
- Petrazhickaya EA. [Foreign experience of accompanying a family in a situation of perinatal loss]. In: Tomilka GS, editor. Actual medical and psychological problems of modern society: materials of the second international scientific and practical conference. Habarovsk: DVGMU; 2015. p. 124-9. Russian.
- Pirogova VІ, Shurpyak SO. [Fetal loss syndrome: from science to practice]. Zdorovia Ukrainy XXI storіchchia: medichna gazeta. 2014;1(13):16-17. Ukrainian. Available from: https://www.health-ua.com/pics/pdf/ZU_2014_Akusher_1/16-17.pdf
- Puz IV. [Perinatal loss as a crisis situation of a woman's personality development]. International scientific and practical conference "Personality in crisis conditions and critical life situations"; 2018 Feb 22-23; Sumy, Ukraine. Sumy: SumDPU im. A.S. Makarenka; 2018. p. 294-6.
- Tutchenko TM. Pregnancy loss. Reproduktivna endokrinologiya. 2015;2:62-64. Ukrainian. Available from: http://reproduct-endo.com/article/view/46934/43105
- Yakzhik IM. [Psychological support of perinatal losses in the hospital]. Scientific works of the Republican Institute of Higher Education. Historical and psychological-pedagogical sciences. 2015;15(2):237-44. Russian. Available from: https://elib.bsu.by/handle/123456789/148532
- Abbaspoor Z, Razmju P, Hekmat K. Relation between quality of life and mental health in pregnant women with prior pregnancy loss. The Journal of Obstetrics and Gynecology Research. 2016;42(10):1290-6. doi: https://doi.org/10.1111/jog.13061
- Early pregnancy loss. American College of Obstetricians and Gynecologists. 2018;132:e197-e207. doi: https://doi.org/10.1097/AOG.0000000000002899
- Asselmann E, Kunas S, Wittchen H, Martini J. Maternal personality, social support, and changes in depressive, anxiety, and stress symptoms during pregnancy and after delivery: A prospective longitudinal study. PLoS One. 2020;15(8).
doi: https://doi.org/10.1371/ journal.pone.0237609
- Bicking Kinsey C, Baptiste-Roberts K, Zhu J, Kjerulff KH. Effect of previous miscarriage on depressive symptoms during subsequent pregnancy and postpartum in the first baby study. Maternal and Child Health Journal. 2015;19(2):391-400.
- Dean E. Miscarriage and ectopic pregnancy. Emergency nurse: the journal of the RCN Accident and Emergency Nursing Association. 2019;27(4):9. doi: https://doi.org/7748/en.27.4.9.s7
- El Hachem H, Crepaux V, May-Panloup P, Descamps P, Legendre G, Bouet P-E. Recurrent pregnancy loss: Current perspectives. International Journal of Women’s Health. 2017;9:331-45.
doi: https://doi.org/10.2147/ ijwh.S100817
- Farren J, Jalmbrant M, Ameye L, Joash K, Mitchell-Jones N, Tapp S, Timmerman D. Post-traumatic stress, anxiety and depression following miscarriage or ectopic pregnancy: A prospective cohort study. British Medical Journal Open. 2016;6(11):e011864. doi: https://doi.org/10.1136/bmjopen-2016-011864
- Harrison S, Alderdice Challenges of defining and measuring perinatal anxiety. Journal of Reproductive and Infant Psychology. 2020;38(1):1-2. doi: https://doi.org/10.1080/02646838.2020.1703526
- Hendson L, Davies D. Supporting and communicating with families experiencing a perinatal loss. Paediatr Child Health. 2018;23(8):549. doi: https://doi.org/10.1093/pch/pxy134
- Horesh D, Nukrian M, Bialik Y. To lose an unborn child: Post-traumatic stress disorder and major depressive disorder following pregnancy loss among Israeli women. General Hospital Psychiatry. 2018;53:95-100. doi: https://doi.org/10.1016/j.genhosppsych.2018.02.003
- Lazarides C, et al. The association between history of prenatal loss and maternal psychological state in a subsequent pregnancy: an ecological momentary assessment (EMA) study. Psychological Medicine. 2021:1-11. doi: https://doi.org/10.1017/S0033291721002221
- Pines D. Pregnancy, miscarriage and abortion. The Contemporary Freudian Tradition. [Internet]. 2020. Available from: https://www.taylorfrancis.com/chapters/edit/10.4324/9781003039495-10/pregnancy-miscarriage-abortion-dinora-pines
- Prabha S Chandra, Madhuri H Nanjundaswamy. Pregnancy specific anxiety: an under-recognized problem. World psychiatry: official journal of the World Psychiatric Association (WPA). 2020;19(3):336-7. doi: https://doi.org/1002/wps.20781
- Dryer R, Brunton R, editors. Pregnancy-Related Anxiety. Theory, Research, and Practice; 2021. doi: https://doi.org/10.4324/9781003014003
- Richards J, Graham R, Embleton N, Campbell C, Rankin J. Mothers’ perspectives on the perinatal loss of a co-twin: A qualitative study. BMC Pregnancy Childbirth. 2015;15:143.
- Sundermann AC, Hartmann KE, Jones SH, Torstenson ES, Velez Edwards DR. Interpregnancy interval after pregnancy loss and risk of repeat miscarriage. Obstetrics & Gynecology. 2017;130(6):1312-8. doi: https://doi.org/10.1097/AOG.0000000000002318
Kryshinska T.Y., Sharun A.V., Zakharov S.V., Stepanskyi D.A. Enghlish-language educational models in medical university
Authors and affiliations
Kryshinska T.Y. Dnipro State Medical University, Dnipro, Ukraine
Sharun A.V. Dnipro State Medical University, Dnipro, Ukraine
Zakharov S.V. Dnipro State Medical University, Dnipro, Ukraine
Key words: content and language integrated learning, English as a medium of instruction, higher medical education, foreign language competence
Abstract. Enghlish-language educational models in medical university. Kryshinska T.Y., Sharun A.V., Zakharov S.V., Stepanskyi D.A. The article considers two approaches to training in high medical school: 1) using English as a Medium of Instruction and 2) Content and Language Integrated Learning the emergence of which was related to a wide spread of bilingualism in society, importance of increase in competitiveness of graduates of domestic universities on international labor market. The authors present the models of teaching within the stated approaches which promote the efficiency of professional training of future doctors in solving professional problems on the basis of implementation of foreign language competence. The paper defines the conditions of content and language integrated models implementation into the learning process. In conclusion, it is noted that the integration of subject and language experience contributes to the optimization of foreign language training and significant improvement of the quality of the educational process. The most pressing issues requiring additional research are identified.
- Bardov VH. [Hygiene and Ecology: a textbook for students of higher medical schools]. Vinnytsia: Nova Knyha; 2018. Ukrainian. Available from: https://nk.in.ua/pdf/1580r.pdf
- Biletska EM. [Terminology Dictionary on Hygiene and Ecology (Ukrainian-Russian-English)]. Dnipro: Akcent PP; 2019. ISBN 978-966-921-228-3. Ukrainian.
- Ball P, Kelly K, Clegg J. Putting CLIL into practice. Oxford: Oxford University Press; 2015. doi: https://doi.org/10.1093/elt/ccx063
- Huertas-Abril C, Shashken A. Exploring the Potential of CLIL in Kazakhstan: A Qualitative Study. Revista Complutense de Educación. 2021;32(2):261-71. doi: https://doi.org/10.5209/rced.68345
- Hemmi C, Banegas D. International perspectives on CLIL. Cham; 2021. doi: https://doi.org/10.1007/978-3-030-70095-9
- Lasagabaster D, Doiz A. Language use in English-medium instruction at university. NY: Taylor&Francis; 2021. doi: https://doi.org/10.1080/13670050.2015.1080658
- Macaro E. Exploring the role of language in English medium instruction. International Journal of Bilingual Education and Bilingualism. 2019;23(3):263-76. doi: https://doi.org/10.1080/13670050.2019.1620678
- Shyrobokov VP. Medical microbiology, virology and immunology: a textbook for English-speaking students of higher medical schools. Vinnytsia: Nova Knyha; 2019. Available from: https://nk.in.ua/pdf/1659.pdf
- Rose H, Curle S, Aizawa I, Thompson G. What drives success in English medium taught courses? The interplay between language proficiency, academic skills, and motivation. Studies in Higher Education. 2019;45(11):2149-61. doi: https://doi.org/10.1080/03075079.2019.1590690
- Shevchenko IV, Kordyuk OM. The methodology of content and language integrated learning (CLIL) in modern methodical and scientific literature of native and foreign scholars. Science and Education a New Dimension. Pedagogy and Psychology. 2018:VI 67(163):51-53. doi: http://doi.org/10.31174/SEND-PP2018-163VI67-12
- Villabona N, Cenoz J. The integration of content and language in CLIL: a challenge for content-driven and language-driven teachers. Language, Culture and Curriculum. 2021 Apr 05;1-15. doi: https://doi.org/10.1080/07908318.2021.1910703
Kolesnyk T.V., Fursa O.V. Features of the daily profile of blood pressure, taking into account polymorphism of the angiotensin II type 1 receptor gene in patients with arterial hypertension after myocardial infarction
Key words: arterial hypertension and myocardial infarction, daily blood pressure profiles, angiotensin II type 1 receptor A1166C polymorphism
Abstract. Features of the daily profile of blood pressure, taking into account polymorphism of the angiotensin II type 1 receptor gene in patients with arterial hypertension after myocardial infarction. Kolesnyk T.V., Fursa O.V. The article presents the results of a study of the daily blood pressure profile, taking into account the polymorphism of the angiotensin II type 1 receptor gene in patients with arterial hypertension with past myocardial infarction. The study involved 36 men with stage III hypertension, grade 1-3 arterial hypertension, with past myocardial infarction. The average age in the study group was 57.0 (52.0; 64.0) years, the experience of hypertension was 6.3 (4.0; 15.0) years. The average period after a heart attack was 48.0 (12.0; 144.0) months. The study of the type 1 angiotensin II receptor gene on the DNA-sorb-B test system was carried out by the polymerase chain reaction method with the restriction of products by endonuclease. 24-hour blood pressure monitoring was performed according to the standard method. It was found that, in general, in the group, the levels of systolic and diastolic blood pressure according to the median corresponded to the target ones. But the blood pressure load indicators both for the day and separately during the day and at night exceeded the normal range. In an intergroup analysis, significantly higher blood pressure figures and pressure load indices were registered in patients with the CC genotype of the angiotensin II type 1 receptor gene. In addition, this group of patients showed a high incidence of adverse variants of circadian fluctuations in blood pressure. In accordance to the daily monitoring data, only 13 (34.2%) patients achieved the target level of blood pressure: with the AA genotype – in 6 (15.9%) patients, with the AC genotype – in 6 (15.9%) patients and in 1 (2.6%) patient with CC genotype of the angiotensin II type 1 receptor gene. In 4 (10.5%) patients with the AA genotype and in 3 (7.9%) patients with the AC genotype of the angiotensin II type 1 receptor gene, achievement of the target level of blood pressure was registered in the normal range of blood pressure load indices. In other cases, the target level of arterial pressure was accompanied by an increase in pressure load indices, which indicates incomplete daily control of arterial hypertension.
- Kovalenko VM, Kornatsky VM, editors. [The state of health of the people of Ukraine and medical care of the tertiary level]. Kyiv; 2019. Ukrainian.
- Semianiv MM. [Risk factors and AGTR1 gene polymorphism (1166A>C) in patients with essential hypertension]. Bukovynian Medical Bulletin. 2020;24(4(96):99-100.
- Bryan W, Mancia G, Spiering W, Agabiti RE, Azizi M, Burnier M, et al. 2018 ESC/ESH Guidelines for the management of arterial hypertension. The Task Force for the management of arterial hypertension of the European Society of Cardiology (ESC) and the European Society of Hypertension (ESH). European Heart Journal. 2018;39(33):3021-4. doi: https://doi.org/10.1093/eurheartj/ehy339
- Holmes L, Lim A, Comeaux CR, Dabney KW, Okundaye O. DNA Methylation of Candidate Genes (ACE II, IFN-γ, AGTR 1, CKG, ADD1, SCNN1B and TLR2) in Essential Hypertension: A Systematic Review and Quantitative Evidence Synthesis. Int J Environ Res Public Health.2019;16(23):4829. doi: https://doi.org/10.3390/ijerph16234829
- ManosroiW, Williams Genetics of Human Primary Hypertension: Focus on Hormonal Mechanisms. Endocr Rev. 2019 Jun;40(3):825-6. doi: https://doi.org/10.1210/er.2018-00071
- Zaitseva MM. [Features of treatment of patients with arterial hypertension with concomitant non-alcoholic fatty liver disease: the role of A1166C polymorphism of angiotensin II receptor gene type 1]. Medicine today and tomorrow. 2014;4(65):50- 58. Ukrainian.
- Saik OV, Demenkov PS, Ivanisenko TV, et Novel candidate genes important for asthma and hypertension comorbidity revealed from associative gene networks. BMC Medical Genomics. 2018;11(1):15. doi: https://doi.org/10.1186/s12920-018-0331-4
- Kamat MA, Blackshaw JA, Young R, et al. PhenoScanner v2: an expanded tool for searching human genotype-phenotype associations. Bioinformatics. 2019;35(22):4851-3. doi: https://doi.org/10.1093/bioinformatics/btz469
- 2021 ESC Guidelines on cardiovascular disease prevention in clinical practice: Developed by the Task Force for cardiovascular disease prevention in clinical practice with representatives of the European Society of Cardiology and 12 medical societies With the special contribution of the European Association of Preventive Cardiology (EAPC). European Heart Journal. 2021 Sep 7;42(34):3227-337. doi: https://doi.org/10.1093/eurheartj/ehab484
- Adapted from Juhani Knuuti, William Wijns and all, 2019 ESC Guidelines for the diagnosis and management of chronic coronary syndromes. The European Society of Cardiology. 2019. Reprinted from the Ukrainian Journal of Cardiology. 2020;(1). Ukrainian. Available from: http://ucardioj.com.ua/index.php/UJC/article/view/209
- [Recommendations of the Association of Cardiologists of Ukraine for the diagnosis and treatment of chronic heart failure]. Kyiv; 2017. Ukrainian. Available from:https://strazhesko.org.ua/upload/rekomendaciyi-hsn-a6-ost.pdf
- Baigent C, Mach F, Catapano AL, et al. ESC/EAS Guidelines for the management of dyslipidaemias: lipid modification to reduce cardiovascular risk. Eur. Heart J. 2019 Aug 31. doi: https://doi.org/10.1093/eurheartj/ehz455
- [Unified clinical protocol of primary, secondary (specialized) and tertiary (highly specialized) medical care: Stable ischemic heart disease]. Kyiv; 2021. Ukrainian. Availablefrom: https://moz.gov.ua/uploads/7/35063-dn_2857_23_12_2021_dod.pdf
- [Kit for DNA extraction from various biological preparations, 100 µl. Instruction NeoPrep50 DNA]. Kyiv; 2013. Russian.
- [Mathematical processing and analysis of biomedical data. 2nd ed.]. Kyiv: MIC "Medinform"; 2018.
- Erbas T, Cinar N, Dagdelen S, et al. Association between ACE and AGT polymorphism and cardiovascular risk in acromegalic patients. Pituitary. 2017;20(5):569-77. doi: https://doi.org/10.1007/s11102-017-0819-5
- Kolesnik TV, Egorov KJ, Kosovo AA. [Analysis of the influence of the features of the daily blood pressure profile on the structural and functional state of the heart in patients with stage II arterial hypertension depending on the I/D polymorphism of the ACE gene]. Cardiology: from science to practice. 2018;31(2):56-66. Ukrainian. doi: https://doi.org/10.30702/card:sp.2018.06.031/s1
- Pierdomenico SD, Pierdomenico AM, Coccina F, Lapenna D, Porreca E. Prognostic Value of Nondipping and Morning Surge in Elderly Treated Hypertensive Patients With Controlled Ambulatory Blood Pressure. Am J 2017 Feb;30(2):159-65. doi: https://doi.org/10.1093/ajh/hpw145
- [Hypertension. Clinical guidelines. Association of Cardiologists of Ukraine. State Expert Center of the Ministry of Security of Ukraine]. Kyiv; 2017. Ukrainian. Availablefrom: https://www.dec.gov.ua/wp-content/uploads/2019/11/kn_artergipert.pdf
- Deepak N Parchwani, Digisha D Patel, Jairam Rawtani, Dharmveer Yadav. Analysis of Association of Angiotensin II Type 1 Receptor Gene A1166C Gene Polymorphism with Essential Hypertension. Indian J Clin Biochem. 2018 Jan;33(1):53-60. doi: https://doi.org/10.1007/s12291-017-0644-7
Koteliukh M.Yu. A model for predicting acute heart failure in patients with acute myocardial infarction by taking into account energy and adipokine metabolism indicators
Key words: adipokine, energy homeostasis, myocardial infarction, heart failure, prognosis
Abstract. A model for predicting acute heart failure in patients with acute myocardial infarction by taking into account energy and adipokine metabolism indicators. Koteliukh M.Yu. Acute heart failure (AHF) is one of the most common complications of acute myocardial infarction (AMI). Currently, adropin, irisin, fatty acid binding protein 4 (FABP 4) and C1q/TNF-binding protein 3 (CTRP 3) are considered to be valid markers of energy homeostasis and the adipokine system in AMI patients. The aim of the study was to predict the development of AHF in AMI patients by taking into account indicators of energy homeostasis and adipokine system using generalized linear mixed model. The study examined 189 patients with acute ST-segment elevation myocardial infarction. Concentrations of adropin, irisin, insulin, FABP4 and CTRP3 were determined by enzyme-linked immunosorbent assay. Fasting capillary blood glucose level was measured by glucoseoxidase method. Insulin resistance (IR) was assessed using homeostasis model assessment of insulin resistance (HOMA-IR). The study identified thrombolysis in myocardial infarction (TIMI) grade before intervention. A generalized linear mixed model was used to develop a method for predicting AHF in AMI patients. The study showed decreased levels of adropin, irisin and CTRP3 and increased levels of FABP4 in AMI patients. A mathematical model for predicting AHF development in AMI patients was proposed. The model consisted of fixed effects, namely, two one-factor indicators (HOMA-IR and systolic blood pressure (BP)), one two-factor indicator (systolic and diastolic BP) and one three-factor indicator (adropin, irisin and CTRP3) and random effects such as four one-factor indicators (FABP4, TIMI Grade Flow, platelets, total cholesterol). The accuracy of predicting the absence of Killip class I AHF was 100%, Killip class II AHF – 0%, Killip class III AHF – 11%, Killip class IV AHF – 82%. It should be noted that systolic BP on day 1 was a strong negative prognostic factor, while HOMA-IR, the combined effect of adropin, irisin and CTRP3, the combined effect of systolic and diastolic BP were positive prognostic factors. Thus, the model showed a very high sensitivity in predicting Killip class IV AHF. The overall accuracy of the model was 89.4%.
- Zaremba YeKh, Salo VM, Myzak YaV, Zaremba OV. [Clinical course of acute myocardial infarction complicated by acute left ventricular failure after coronary angiography with stenting]. Family medicine. 2016;3:30-32. Ukr. doi: https://doi.org/10.30841/2307-5112.3(65).2016.79966
- Kravchun PH, Shaparenko OV. [Irisin as a marker of insulin resistance in patients with hypertension and obesity]. Actual problems of modern medicine: Bulletin of Ukrainian medical stomatological academy. 2018;18(1):68-70. Available from: http://elib.umsa.edu.ua/bitstream/umsa/11045/1/ Kravchun_Irisin_as_a_marker_ of_insulin_resistance_in_patients.pdf
- Kupnovytska IG, Ersteniuk AM. [Laboratory diagnostics]. Vinnytsia: Nova Knyha; 2017. Ukrainian.
- Solomonchuk AV, Rasputina LV. [Prevalence dynamics, sex-age characteristics and mortality in acute heart failure in patients with myocardial infarction]. Reports of Vinnytsia national medical university. 2020;24(2):303-8. Ukrainian.
- Abd El-Mottaleb N, Galal HM, El Maghraby KMA, Gadallah AI. Serum irisin level in myocardial infarction patients with or without heart failure. Canadian Journal of Physiology and Pharmacology. 2019;97(10). doi: http://doi.org/10.1139/cjpp-2018-0736
- Ertem AG, Unal S, Efe TH, Acar B, Yayla C, Kuyumcu MS, et al. Association between serum adropin level and burden of coronary artery disease in patients with non-ST elevation myocardial infarction. Anatolisn journal of 2017;17(2):119-24. doi: http://doi.org/10.14744/AnatolJCardiol.2016.7149
- YildirimA, Sumbul HE, Koca H, Kucukosmanoglu M, Icen YK, Koc Complement C1q/tumor necrosis factor-related protein-3 (CTRP3) is significantly decreased in patients with heart failure and closely related with ventricular tachycardia. Acta Cardiologica Sinica. 2021;37(3):278-85. doi: http://doi.org/10.6515/ACS.202105_37(3).20201019B
- Obokata M, Iso T, Ohyama Y, Sunaga H, Kawaguchi T, Matsui H, et al. Early increase in serum fatty acid binding protein 4 levels in patients with acute myocardial infarction. European Heart Journal: Acute Cardiovascular Care. 2016;7(6):561-9. doi: http://doi.org/10.1177/2048872616683635
- Ibanez B, James S, Agewall S, Antunes MJ, Bucciarelli-Ducci C, Bueno H, et al. 2017 ESC Guidelines for the management of acute myocardial infarction in patients presenting with ST-segment elevation. European Heart Journal.2018;39(2):119-77. doi: http://doi.org/10.1093/eurheartj/ehx393
- Rodríguez-Calvo R, Girona J, Alegret JM, Bosquet A, Ibarretxe D, Masana L. Role of the fatty acid-binding protein 4 in heart failure and cardiovascular disease. Journal of Endocrinology. 2017;233(3):R173-84. doi: http://doi.org/10.1530/joe-17-0031
- ShenS, Gao R, Bei Y, Li J, Zhang H, Zhou Y, et al. Serum irisin predicts mortality risk in acute heart failure patients. Cellular Physiology and Biochemistry. 2017;42(2):615-22. doi: http://doi.org/10.1159/000477867
- Shelest BO. Hypertension grade influence on inflammation and adropin level in diabetic patients with obesity. Ukrainian Journal of medicine, biology and sport. 2019;4(5):244-8.
doi: http://doi.org/26693/ jmbs04.05.244
- SiY, Fan W, Sun A review of the relationship between CTRP family and coronary artery disease. Current Atherosclerosis Reports. 2020;22(6):22. doi: http://doi.org/10.1007/s11883-020-00840-0
- Stroup WW, Milliken GA, Claassen EA, Wolfinger RD. SAS for mixed models: introduction and basic applications. Cary, NC: SAS Institute; 2018.
- Syvolap VD, Kapshуtar NI. Predictors of acute heart failure in patients during the acute period of Q-wave myocardial infarction. Zaporozhye medical journal. 2019;21(2):160-4. doi: http://doi.org/10.14739/2310-1210.2019.2.161319
Hamid Dahmarde, Faramarz Fazeli. Should renal resistive index be considered as a routine predictor in newly diagnosed acute hydronephrosis in patients with unilateral renal colic?
Key words: renal resistive index, renal colic, acute hydronephrosis
Abstract. Should renal resistive index be considered as a routine predictor in newly diagnosed acute hydronephrosis in patients with unilateral renal colic? Hamid Dahmarde, Faramarz Fazeli. Urinary tract stones are the most common causes of urinary tract obstruction, leading to patient hospitalization in the emergency room. This study is aimed to evaluate the use of the mean resistance index (mRI) to predict hydronephrosis in patients admitted for acute renal colic. This prospective study was performed on 100 patients who were admitted to the emergency department (ED) with unilateral renal colic (RC) problem. At the first visit, the following was done for all patients: 1. blood was taken from them for laboratory studies; 2. ECG and cardiological test; 3. plain film of the urinary tract; 4. аbdominal US; 5. bilateral color doppler ultrasound (CDUS) with renal RI measurement. Based on the information collected at different times during the experiment, patients were divided into two groups: the group that showed signs of dilatation (group A) and the group with hydronephrosis (group B). The obtained data were analyzed statistically by SPSS version 20. A mRI with a 0.68 cut off value was the most accurate (AUC:0.878), and Sensitive (90%) cut-off value for prediction of hydronephrosis development among patients presenting with RC and renal stone. This cut-off value also provided the highest positive (94%) and negative (76%) predictive values. CDUS with RI measurement is an easy and non-invasive method that provides useful information about urinary tract obstruction stones in patients with unilateral RC. In our study, this method was able to predict successfully the onset of acute renal dilatation. This was done with different levels of sensitivity, specificity, accuracy and diagnostic efficiency that were higher than ultrasound. Daily use of CDUS in ED can improve ultrasound, especially when other methods are contraindicated. For example, the methods by which the patient is exposed to radiation (pregnancy, allergies, renal failure, etc.) or intravenous injection of contrast agents (urography and CT
- Hall J, Linton KD. Obstruction of the upper and lower urinary tract. Surgery (Oxford). 2008;26:197-202. doi: https://doi.org/10.1016/j.mpsur.2008.03.007
- Pepe P, Motta L, Pennisi M, Aragona F. Functional evaluation of the urinary tract by color-Doppler ultrasonography (CDU) in 100 patients with renal colic. Eur J Radiol. 2005;53:131-5. doi: https://doi.org/10.1016/j.ejrad.2004.01.014
- Rishi N, Anil KS, Kaza RCM, Anand VJ. The obstructed kidney. Indian J Surg. 2005;67:21-8.
- Thomas A, Andrianne R. Excruciating flank pain: “acute renal colic”. Rev Med Liege. 2004;59:215-20.
- Fauci AS, Braunwald E, Isselbacher KJ, Wilson JD, Martin JB, Kasper DL, et al. Harrison’s principles of internal medicine. 14th ed. New York, NY: McGraw-Hill Book Co; 1998.
- Ramello A, Vitale C, Marangella M. Epidemiology of nephrolithiasis. J Nephrol. 2000;13:S65-70.
- Robertson WG, Hughes H. Epidemiology of urinary stone disease in Saudi Arabia. In: Ryall R, Bais R, Marshall VR, Rofe AM, Smith LH, Walker VR, eds. Urolithiasis 2. Plenum Press, New York: London; 1994:453-5. doi: https://doi.org/10.1007/978-1-4615-2556-1_174
- Tamm EP, Silverman PM, Shuman WP. Evaluation of the patient with flank pain and possible ureteral calculus. Radiology.2003;228:319-29. doi: https://doi.org/10.1007/s00240-007-0116-2
- Onur MR, Cubuk M, Andic C, Kartal M, Arslan G. Role of resistive index in renal colic. Urol Res. 2007;35:307-12.
- Leipe K, Taut-Sack H. Can renal Doppler sonography replace diuretic radionuclide renography in infants with hydronephrosis? Turk J Pediatr. 2006;48:221-7. doi: https://doi.org/10.1046/j.1464-410x.1999.00946.x
- Shokeir AA, Abdulmaaboud M. Resistive index in renal colic: a prospective study. BJU international. 1999 Mar 1;83(4):378-82. doi: https://doi.org/10.1136/emj.2005.028589
- Kartal M, Eray O, Erdogru T, Yilmaz S. Prospective validation of a current algorithm including bedside US performed by emergency physicians for patients with acute flank pain suspected for renal colic. Emergency medicine journal. 2006 May 1;23(5):341-4.
- aza Perez JA, Ferrer JF, Urdangarain OO, Nunez AB. Renal colic at emergency departments. Epidemiologic, diagnostic and etiopathogenic study. Archivos espanoles de urologia. 2010 Apr 1;63(3):173-87. doi: https://doi.org/10.2147/IJNRD.S27628
- Sayani MA, Shazlee K, Hamid RS, Hamid K. Functional evaluation of the urinary tract by duplex Doppler ultrasonography in patients with acute renal colic. International journal of nephrology and renovascular disease. 2012;5:15. doi: https://doi.org/10.1017/S1481803500012240
- Edmonds ML, Yan JW, Sedran RJ, McLeod SL, Theakston KD. The utility of renal ultrasonography in the diagnosis of renal colic in emergency department patients. Canadian Journal of Emergency Medicine. 2010 May;12(3):201-6.
- Kavakli HS, Koktener A, Yilmaz A. Diagnostic value of renal resistive index for the assessment of renal colic. Singapore medical journal. 2011 Apr 1;52(4):271. doi: https://doi.org/10.1007/s003300050568
- Opdenakker L, Oyen R, Vervloessem I, Goethuys H, Baert AL, Baert LV, Marchal G. Acute obstruction of the renal collecting system: the intrarenal resistive index is a useful yet time-dependent parameter for diagnosis. European radiology. 1998 Sep;8(8):1429-32. doi: https://doi.org/10.1148/radiology.186.3.8430174
- Platt JF, Rubin JM, Ellis JH. Acute renal obstruction: evaluation with intrarenal duplex Doppler and conventional US. Radiology. 1993;186:685-8. doi: https://doi.org/10.1148/radiology.171.2.2649925
- Platt JF, Rubin KM, Ellis JH, DiPietro M. Duplex Doppler US of the kidney: Differentiation of obstructive from non-obstructive dilatation. Radiology. 1989;171:515-7. doi: https://doi.org/10.1046/j.1464-410X.2000.00583.x
- Webb JA. Ultrasonography and Doppler studies in the diagnosis of renal obstruction. BJU Int. 2000;86:25-32. doi: https://doi.org/10.1259/0007-1285-65-771-207
- Rodgers PM, Bates JA, Irving HC. Intrarenal Doppler ultrasound studies in normal and acutely obstructed kidneys. Br JRadiol. 1992;65:207-12.
- Moody TE, Vaughan ED Jr, Gillenwater JY. Relationship between renal blood flow and ureteral pressure during 18 hours of total unilateral occlusion: implications for changing sites of increased renal resistance. Invest Urol.1975;13:246-51.
- Ryan PC, Macer KP, Murphy B. Experimental partial obstruction: pathophysiological changes in upper tract pressures and renal blood flow. J Urol. 1987;138:674-8. doi: https://doi.org/10.7863/jum.19184.108.40.2063
- Lee HJ, Kim SH, Jeong YK. Doppler sonographic resistive index in obstructed kidneys. J Ultrasound Med. 1996;15:613-8.
- Klahr S. Pathophysiology of obstructive nephropathy: a 1991 update. Semin Nephrol. 1991;11:156-68.
- Croan JJ. Acute urinary tract obstruction in adults. In: RSNA Categorical Course in Genitourinary Radiology. Oak Brook, IL: Radiological Society of North America; 1994:103-108.
- Platt JF. Urinary obstruction. Radiol Clin North Am. 1996;34:1113-29.
- Vaughan ED Jr, Sorenson EJ, Gillenwater JY. The renal hemodynamic response to chronic unilateral complete ureteral occlusion. Invest Urol. 1970;8:78-90. doi: https://doi.org/10.1046/j.1464-410x.1999.00946.x
- Shokeir AA, Abdulmaaboud M. Resistive index in renal colic: a prospective study. BJU Int. 1999;83:378-82. doi: https://doi.org/10.1007/s003300050568
- Opdenakker L, Oyen R, Vervloessem I, Goethuys H, Baert AL, Baert LV, et al. Acute obstruction of the renal collecting system: the intrarenal resistive index is a useful yet time-dependent parameter for diagnosis. Eur Radiol.1998;8:1429-32. doi: https://doi.org/10.1016/S0022-5347(05)66431-2
- Shokeir AA, Abdulmaaboud M. Prospective comparison of nonenhanced helical computerized tomography and Doppler ultrasonography for the diagnosis of renal colic. J 2001;165:1082-4. doi: https://doi.org/10.1016/S0022-5347(05)66316-1
- Rawashdeh YF, Djurhuus JC, Mortensen J, Hørlyck A, Frokiaer J. The intrarenal resistive index as a pathophysiological marker of obstructive uropathy. J Urol. 2001;165:1397-404.
- Sauvain JL, Pierrat V, Chambers R, Bui Xuan P, Palascak P, Boursheid D, et al. Echography and pulsed Doppler of the arteries of the renal parenchyma in obstructive syndromes and dilatation of the excretory cavities of the kidney. J Radiol. 1989;70:389-98. doi: https://doi.org/10.1016/0720-048X(96)01055-8
- De Toledo LS, Martinez-Berganza AT, Cabrejas CR, de Gregorio AMA, Cortina PP, Saldias RL. Doppler duplex ultrasound in renal colic. Eur J Radiol. 1996;23:143-8. doi: https://doi.org/10.2147/IJNRD.S27628
- Sayani R, Ali M, Shazlee K, Hamid RS, Hamid K. Functional evaluation of the urinary tract by duplex Doppler ultrasonography in patients with acute renal colic. Int J Nephrol Renovasc Dis. 2012;5:15e21. doi: https://doi.org/10.1023/B:UROL.0000020302.65486.f8
- Haroun A. Duplex Doppler sonography in patients with acute renal colic: prospective study and literature review. Int Urol Nephrol. 2003;35:135e40. doi: https://doi.org/10.1016/j.jus.2012.10.003
- Piazzese EMS, Mazzeo GI, Galipo S, Fiumara F, Canfora C, Angiom LG. The renal resistive index as a predictor of acute hydronephrosis in patients with renal colic. J Ultrasound. 2012;15:239e246.
Sadullayev A.S., Medvediev M.V. Effect of laparoscopic excision of the endometriotic cyst capsule on the ovarian reserve (a retrospective analysis)
Key words: endometriotic cyst, AMH, Anti-Müllerian hormone, ovarian reserve, laparoscopy, excision of the cyst capsule
Abstract. Effect of laparoscopic excision of the endometriotic cyst capsule on the ovarian reserve (a retrospective analysis). Sadullayev A.S., Medvediev M.V. Endometriotic cyst is one of manifestations of the endometriotic process and its incidence among all women with endometriosis varies from 17 to 44%. This chronic process in the ovary is accompanied by persistent inflammation, which leads to fibrosis of the ovarian cortex and loss of follicles. Laparoscopic excision of the endometriotic cyst capsule is by far the mainstay of treatment for cysts of 3 cm or more in diameter. This standard surgical procedure can reduce an ovarian reserve marker such as anti-Müllerian hormone (AMH), raising concerns about reduced fertility after surgery. The aim of the study was to analyze the effect of operative, in particular, laparoscopic cystectomy, on the ovarian reserve in women of fertile age with endometriotic cysts. A retrospective analysis of the data of 202 women with endometriotic cysts who underwent examination, surgery and outpatient observation from 2011 to 2020 was carried out. The analysis was carried out in comparison before and after surgical treatment of all cases. The state of the ovarian reserve was assessed by the level of serum AMH. At baseline, the mean AMH in women with bilateral cysts 1,78 (95% CI 1,62; 1,91) ng/ml was lower than in women with monolateral cysts 2,44 (95% CI 2,18; 2,54) ng/m (p<0.001). When comparing AMH values 6 months after surgery, it was found that in all women the median AMH level significantly decreased by an average of 0,34 (95% CI 0,2-0,48) ng/ml (p<0,001). Laparoscopic excision of the cyst capsule is the main method of treatment of endometriotic cysts, and its minimally invasiveness and acceptability of use in women of fertile age makes it the standard surgical approach. However, the analysis showed that, nevertheless, laparoscopic excision of the endometriotic cyst capsule does not guarantee the safety of the negative effect of this method on the ovarian reserve, both during and after its application, on the contrary, significantly reducing the AMH values in comparison with its levels before surgery.
- Andriiets A, Yuzko O. [Number of antral follicles as a marker of ovarian reserve in patients with infertility in ovarian endometriosis]. Neonatology, surgery and perinatal medicine. 2018;8(4):43-46. Ukrainian. doi: https://doi.org/10.24061/2413-4260.VIII.4.30.2018.8
- Feskov VО. [Modern approaches to the treatment of infertility in women with ovarian endometriosis, using in vitro fertilization]. Scientific Journal «ScienceRise: Medical Science». 2017;15(7):39-43. Ukrainian.
- Gruzeva TS, Lekhan VM, Ognev VA, et al. [Biostatistics: a textbook]. Gruzeva TS, editor. Vinnytsia: Nova Knyha; 2020. p. 47-138. Ukrainian.
- Cranney R, Condous G, Reid S. An update on the diagnosis, surgical management, and fertility outcomes for women with endometrioma. Acta Obstet Gynecol Scand. 2017;96(6):633-43. doi: https://doi.org/10.1111/aogs.13114
- Demirdag E, Guler I, Selvi I, Cevher Akdulum MF, Canan S, Erdem A, Erdem M. Analysis of 2438 cycles for the impact of endometrioma and its surgery on the IVF outcomes. Eur J Obstet Gynecol Reprod Biol. 2021;(263):233-8. doi: https://doi.org/10.1016/j.ejogrb.2021.06.034
- Kitajima M, Khan KN, Harada A, Taniguchi K, Inoue T, Kaneuchi M, Miura K, Masuzaki H. Association between ovarian endometrioma and ovarian reserve Front Biosci (Elite Ed). 2018;1(10):92-102. doi: https://doi.org/10.2741/e810
- Karadağ C, Demircan S, Turgut A, Çalışkan E, Turk J. Effects of laparoscopic cystectomy on ovarian reserve in patients with endometrioma and dermoid cyst. Obstet 2020;17(1):15-20. doi: https://doi.org/10.4274/tjod.galenos.2020.37605
- Matalliotakis M, Goulielmos GN, MatalliotakiC, Trivli A, Matalliotakis I, Arici A. Endometriosis in Adolescent and Young Girls: Report on a Series of 55 Cases. J Pediatr Adolesc Gynecol. 2017;30(5):568-70. doi: https://doi.org/10.1016/j.jpag.2017.05.007
- Gordts S, Koninckx P, Brosens I. Pathogenesis of deep endometriosis. Fertil Steril. 2017:108(6):872-85.e1. doi: https://doi.org/10.1016/j.fertnstert.2017.08.036
- Petrie A, Sabin C. Medical Statistics at a Glance, 4th Ed. Wiley-Blackwell; 2019.
- Lee SY, Kim Mi-La, Seong SJu, Bae JW, Cho J Recurrence of Ovarian Endometrioma in Adolescents after Conservative, Laparoscopic Cyst Enucleation. Pediatr AdolescGynecol. 2017;30(2):228-33. doi: https://doi.org/10.1016/j.jpag.2015.11.001
- Chapron C, Marcellin L, Borghese B, Santulli P. Rethinking mechanisms, diagnosis and management of endometriosis. Nat Rev Endocrinol. 2019;15(11):666-82. doi: https://doi.org/10.1038/s41574-019-0245-z
- DiVasta AD, Vitonis AF, Laufer MR, Missmer SA. Spectrum of symptoms in women diagnosed with endometriosis during adolescence vs adulthood. Am J Obstet Gynecol. 2018:218(3):324.e1-e11. doi: https://doi.org/10.1016/j.ajog.2017.12.007
- Whittier N, Wildhagen T, Gold H. Statistics for Social Understanding With Stata and SPSS. Rowman & Littlefield Pub. 2019(17)210-45.
- Seo JW, Lee DY, Yoon BK, Choi DJ. The Efficacy of Postoperative Cyclic Oral Contraceptives after Gonadotropin-Releasing Hormone Agonist Therapy to Prevent Endometrioma Recurrence in Adolescents. Pediatr Adolesc Gynecol. 2017:30(2):223-7. doi: https://doi.org/10.1016/j.jpag.2016.10.004
Ostafiichuk S.O., Volosovskiy P.R., Prudnikov P.M., Henyk N.I., Makarchuk O.M. Association of hyperemesis gravidarum with the risk of development of small for gestational age fetus
Authors and affiliations
Volosovskiy P.R. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Prudnikov P.M. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Henyk N.I. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Makarchuk O.M. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Key words: hyperemesis gravidarum, human chorionic gonadotropin, small for gestational age fetus
Abstract. Association of hyperemesis gravidarum with the risk of development of small for gestational age fetus. Ostafiichuk S.O., Volosovskiy P.R., Prudnikov P.M., Henyk N.I., Makarchuk O.M. The goal of this study was to determine the association of hyperemesis gravidarum with the risk of development small for of fetus gestational age (SGA). Materials and methods. There were studied 327 pregnant women. The main group included 218 women with hyperemesis gravidarum, who were divided into two groups: 140 patients who were first hospitalized with hyperemesis gravidarum in the first trimester (up to 12 weeks of pregnancy) and 78 - who were first hospitalized with hyperemesis gravidarum in the second trimester (12-21 weeks of pregnancy). 109 pregnant women without vomiting were at control group. The diagnosis of SGA fetus was established on the basis of standard ultrasonic fetometry on the ALOKA SSD-1700. Determination of human serum chorionic gonadotropin (HCG) levels was performed at 15-20 weeks and was evaluated as MoM. The results were statistically analyzed using Statistica 10 (Serial Number: STA999K347150-W) and MEDCALC®. Results. In the main group, the SGA fetus was 3.4-fold more frequent compared with pregnant women in the control group (9.6% vs. 2.8%, p<0.05; OR=3.77; 95%CI:1,10-12.92). It was found that hyperemesis gravidarum in the first trimester does not affect the deviation of fetal weight deviations (p>0.05). However, hyperemesis gravidarum in pregnant women in the second trimester increases the risk of developing SGA fetus (OR=6.42; 95%CI 1.75-23.62; p<0.01) compared with control. Pregnant women with HCG≥2.5 MoM were 3.0-fold more likely to be diagnosed SGA fetus than with HCG<2.5 MoM (75.0% vs. 25.0%, p<0.001; OR=9.00; 95%CI:1.42-57.12) and 2.3-fold compared with the development of the normal fetus (75.0% vs. 33.3%, p<0.001; OR=6.00; 95%CI:1.47-24,4). Conclusion. Hyperemesis gravidarum in the second trimester of pregnancy and elevation of HCG level in the second trimester (≥2,5 МоМ) can be seen as markers of placental disfunction and high risk for SGA fetus.
- Holovanova IA, Byelikova IV, Liakhova NO. [Basics of medical statistics]. Poltava: Educational manual. 2017:113. Ukrainian.
- Lapovets LY, Lebed HB, Yastremska OO. [Clinical laboratory diagnostics]. 2-d ed. Kyiv: «Medytsyna»; 2021. Ukrainian.
- Ostafiichuk SO. [Assessment of association of the Pro12Ala polymorphism of PPAR-γ gene with the risk of obstetric complications]. Zaporizkyi medychnyi zhurnal. 2019;5(116):631-7. doi: https://doi.org/10.14739/2310-1210.2019.5.179431
- Romanenko TH, Melnychuk IP, Hymenko MV. [Fetal growth retardation (clinical lecture)]. Zdorovya zhinky. 2014;8(94):9-14. Ukrainian.
- Bolin M, Akerud H, Cnattingius S, Stephansson O, Wikstrom A. Hyperemesis gravidarum and risks of placental dysfunction disorders: a population-based cohort study. BJOG.2013;120:541-7. doi: https://doi.org/10.1111/1471-0528.12132
- Burton GJ, Jauniaux E. Pathophysiology of placental-derived fetal growth restriction. Am J Obstet Gynecol. 2018;218(2S):S745-61. doi: https://doi.org/10.1016/j.ajog.2017.11.577
- Fejzo MS, Sazonova OV, Sathirapongsasuti JF, Hallgrímsdóttir IB, Vacic V, MacGibbon KW, et al. Placenta and appetite genes GDF15 and IGFBP7 are associated with hyperemesis gravidarum. Nat Commun. 2018;1(9):1-9.
- FinkenMJJ, van der Steen M, Smeets CCJ, Walenkamp MJE, de Bruin C, Hokken-Koelega ACS, Wit Children born small for gestational age: differential diagnosis, molecular genetic evaluation, and implications. Endocrine Reviews. 2018 Dec;6(39):851-94. doi: https://doi.org/10.1210/er.2018-00083
- Gobbo DGF, Yin Y, Choufani S, ButcherEA, Wei J, Rajcan-Separovic E, et al. Genomic imbalances in the placenta are associated with poor fetal growth. Mol Med. 2021;3(8):27. doi: 10.1186/ s10020-020-00253-4
- Jennings LK, Krywko DM. Hyperemesis gravidarum. [Updated 2020 Nov 21]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan. Available from: https://www.ncbi.nlm.nih.gov/books/NBK532917
- LeesCC, Stampalija T, Baschat AA, Costa F da Silva, Ferrazzi E, Figueras F, Hecher K, Kingdom J, et al. ISUOG Practice Guidelines: diagnosis and management of small-for-gestational-age fetus and fetal growth restriction. Ultrasound Obstet Gynecol. 2020;56:298-312. doi: https://doi.org/10.1002/uog.22134
- Morokuma S, Shimokawa M, Kato K, Sanefuji M, Shibata E, Tsuji M, et al. Relationship between hyperemesis gravidarum and small-for-gestational-age in the Japanese population: the Japan Environment and Children’s Study (JECS). BMC Pregnancy Childbirth. 2016;1(16):247. doi: https://doi.org/10.1186/s12884-016-1041-6
- Sharony R, Zipper O, Amichay K, WiserA, Kidron D, Biron-Shental T, Maymon The magnitude of elevated maternal serum human chorionic gonadotropin and pregnancy complications. J Obstet Gynaecol. 2017;5(37):576-9. doi: https://doi.org/10.1080/01443615.2016.1268578
- Thalava K, Manmatharaj, Prabhakar ER, Vaithialingam A. Human chorionic gonadotropin (Hcg) – levels in pre-eclampsia and hyperemesis. Global J Res Analysis.2017 Feb;2(6):48-51. doi: https://doi.org/10.36106/gjra
- Veenendaal MV, Van Abeelen AF, Painter RC, van der Post JA, Roseboom TJ. Consequences of hyperemesis gravidarum for offspring: a systematic review and meta-analysis. BJOG.2011;118:1302-13. doi: https://doi.org/10.1111/j.1471-0528.2011.03023.x
- Vikanes ÅV, Støer NC, Magnus P, Grjibovski AM. Hyperemesis gravidarum and pregnancy outcomes in the Norwegian mother and child cohort – a cohort study. BMC Pregnancy and Childbirth. 2013;13:169-177. doi: https://doi.org/10.1186/1471-2393-13-169
- Yeaton-Massey A, Baer RJ, Rand L, Jelliffe-Pawlowski LL, Lyell DJ. Adverse pregnancy outcomes by degree of maternal serum analyte elevation: a retrospective cohort study. Am J Perinatol Rep. 2020;10:e369-pe379. doi: https://doi.org/10.1055/s-0040-1716741
Dynnik V.O., Dynnik O.O., Druzhinina A.E. Role of vitamin D in the formation of abnormal uterine bleeding in the pubertate period
Authors and affiliations
Key words: abnormal uterine bleeding, vitamin D, hormonal status, adolescent girls
Abstract. Role of vitamin D in the formation of abnormal uterine bleeding in the pubertate period. Dynnik V.O., Dynnik O.O., Druzhinina A.E. The problem of the participation of vitamin D in the pathogenesis of various diseases has been attracted the attention of scientists in recent years. Vitamin D deficiency is regarded as a global problem that increases the risk of many chronic diseases. More recently, the emphasis has shifted to the non-skeletal effects of vitamin D. A lot of literature suggests that vitamin D plays an important role in the regulation of processes in the ovaries that determine female fertility, female reproductive potential. Vitamin D receptor enzymes involved in its metabolism are expressed in both the central and peripheral reproductive organs (hypothalamic-pituitary system, ovaries, uterus, placenta). All of this suggests that vitamin D is involved in the regulation of menstrual function. The aim of the study is to identify the relationships between gonadotropic and steroid hormones with the content of vitamin D in menstrual disorders in adolescent girls by type of abnormal uterine bleeding (AUB). 167 adolescent girls with AUB were examined. The levels of Luteinizing hormone (LH), Follicle-stimulating hormone (FSH), Prolactin (PRL), total estradiol (E2), testosterone (T), cortisol (C), vitamin D were determined. It was revealed that in 92.1% of girls with AUB have a decrease in the level of vitamin D, and 53.9% of them have a sharp reduce of its. A factorial model of the relationship between pituitary-gonadal hormones and vitamin D, on the basis of which it may be assumed that vitamin D makes a significant contribution to the formation of AUB in adolescence. It participates in the mechanisms of stimulation of gonadotropins, affects the activity of sex hormones through the promoters of receptors located both in the hypothalamic-pituitary region and in the periphery in the ovaries. This is evidenced by factor weights, which serve as analogues of the correlation ratios and show the degree of interconnection between the variables in the factors selected. The changes in the content of vitamin D revealed indicate the need and expediency of its determination in the blood of patients with AUB during puberty for early diagnosis of its abnormalities and timely prevention of serious complications in the future.
- Muscogiuri G, Altieri B, de Angelis C, Palomba S, Pivonello R, Colao A, Orio F. Shedding new light on female fertility: The role of vitamin D. Rev EndocrMetabDisord.2017 Sep;18(3):273-83. doi: https://doi.org/10.1007/s11154-017-9407-2
- Zufarova DSA, Egamberdieva DA. Influence of vitamin D on the formation of the menstrual function in teenage girls.Asian Journal of Multidimensional Research (AJMR).2020;9(3):123-30. doi: https://doi.org/10.5958/2278-4853.2020.00071.3
- Afsane Bahrami, Amir Avan, Hamid Reza Sadeghnia, Habibollah Esmaeili, Maryam Tayefi, Faezeh Ghasemi. High dose vitamin D supplementation can improve menstrual problems, dysmenorrhea, and premenstrual syndrome in adolescents. Gynecological Endocrinology.2018;34(8):659-63. doi: https://doi.org/10.1080/09513590.2017.1423466
- Sabriye Arslan, Yasemin Akdevelioğlu. The Relationship Between Female Reproductive Functions and Vitamin D. Journal of the American College of Nutrition. 2018;37(6):546-51. doi: https://doi.org/10.1080/07315724.2018.1431160
- Łagowska K. The Relationship between Vitamin D Status and the Menstrual Cycle in Young Women: A Preliminary Study. Nutrients. 2018 Nov 11;10(11):1729. PMID: 30423869;PMCID: PMC6265788. doi: https://doi.org/10.3390/nu10111729
- Bashmakova NV, Lisovskaya TV, Vlasova VY. Pathogenetic role of vitamin D deficiency in the development of menstrual dysfunction in pubertal girls: a literature review. Gynecological Endocrinology, 2017;33(sup1):52-55. doi: https://doi.org/10.1080/09513590.2017.1404235
- Drakopoulos P, van de Vijver A, Schutyser V. The effect of serum vitamin D levels on ovarian reserve markers: a prospective cross-sectional study. Hum Reprod. 2017;32:208-14. PubMed, Web of Science, Google Scholar. doi: https://doi.org/10.1093/humrep/dew304
- Charushina MA, Pleshkova KV, Askerova MG. Disorders of the formation of the menstrual cycle in adolescent girls. Topical issues of modern medical science and health care. Collection of research papers; 2019. p. 189-193.
- Ashurova NG, Bobokulova SB. Another look at the problem of juvenile age. Biology integrative medicine. 2020;42(2):95-106.
- Dynnik VA, Shcherbina NA, Dynnik AA. The relationship of individual links of the reproductive system with hormones of energy metabolism in abnormal uterine bleeding in adolescence. Obstetrics and gynecology. 2018;(2):102-7. doi: https://doi.org/10.18565/aig.2018.2.102-107
- Mohamad Irani, ZaherMerhi. Role of vitamin D in ovarian physiology and its implication in reproduction: a systematic review. Fertility and Sterility. 2014;102(2):460-8.e3. doi: https://doi.org/10.1016/j.fertnstert.2014.04.046
- Chu C, Tsuprykov O, Chen X, Elitok S, Krämer BK, Hocher B. Relationship Between Vitamin D and Hormones Important for Human Fertility in Reproductive-Aged Women. Front Endocrinol (Lausanne). 2021 Apr 14;12:666687. doi: https://doi.org/10.3389/fendo.2021.666687
- Gleicher N, Kim A, Weghofer A, et al. Starting and resulting testosterone levels after androgen supplementation determine at all ages in vitro fertilization (IVF) pregnancy rates in women with diminished ovarian reserve (DOR). J Assist Reprod Genet. 2013;30:49-62. doi: https://doi.org/10.1007/s10815-012-9890-z
- Eun Mi Chang, You Shin Kim, Hyung Jae Won, Tae Ki Yoon, Woo Sik Lee. Association Between Sex Steroids, Ovarian Reserve, and Vitamin D Levels in Healthy Nonobese Women. The Journal of Clinical Endocrinology &Metabolism, 2014 July 1;99(7):2526-32. doi: https://doi.org/10.1210/jc.2013-3873
- Harmon QE, Umbach DM, Baird DD. Use of Estrogen-Containing Contraception Is Associated with Increased Concentrations of 25-Hydroxy Vitamin D. J Clin Endocrinol 2016 Sep;101(9):3370-7. doi: https://doi.org/10.1210/jc.2016-1658
- Zaydieva YZ, Balan VE. Vitamin D and women's reproductive health (literature review). Medical Council. 2018;(12):164-72. doi: https://doi.org/10.21518/2079-701X-2018-12-164-172
Nosov S.G., Yuryeva L.М., Shusterman T.Y., Nekrasova O.V. Clinical and pathogenetic prediction of the dynamics of the course of psychoses in epilepsy
Authors and affiliations
Nosov S.G. Dnipro State Medical University, Dnipro, Ukraine
Yuryeva L.М. Dnipro State Medical University, Dnipro, Ukraine
Key words: epileptic psychoses, prognosis, transient course, paroxysmal course, chronic course, clinical-pathogenetic transformation, treatment
Abstract. Clinical and pathogenetic prediction of the dynamics of the course of psychoses in epilepsy. Nosov S.G., Yuryeva L.М., Shusterman T.Y., Nekrasova O.V. Transient epileptic psychoses in 30-40% of cases turn into psychotic states with a long, paroxysmal or chronic course. The objective of this article is to conduct a systematic analysis of modern literature sources to clarify the factors, clinical and pathogenetic patterns of transformation of transient (short-term) epileptic psychosis into psychotic states with prolonged and chronic course, as well as to identify pathogenetically oriented principles of treatment of such patients. The prognostic value of clinical and neurophysiological factors regarding increasing of the psychotic process duration has been noticed, clinical patterns of transformation of short-term psychosis into long-term (changes in the dynamics and structure of psychosis, features of the relationship with organic personality disorder and dementia in epilepsy, as well as epileptic seizures) have been shown. A number of important neurophysiological pathogenetic mechanisms of increasing the psychosis duration (growing cerebral hypofrontality, temporal localization and regular spread of the epileptic process) have been identified. Pathogenetically oriented principles of treatment tactics of patients with epileptic psychoses have been described and analyzed taking into account the revealed regularities of increasing of their duration.
- Avakian GN, Avakian GG. [Transformation of the epileptic system. State of the issue and possibilities for solving the problem]. Epilepsiia i paroksizmalnye sostoianiia.2017;9:6-19. doi: https://doi.org/10.17749/2077-8333.2017.9.2.006-019
- Avakian GN, Belousova ED, Burd SG, Vlasov PN, Ermolenko NA, Kissin MYa, Lebedeva AV, Lipatova LV, Kiteva-Trenchevskaja G. [Epileptology problems. Key priorities, tasks, challenges and ways to address them]. Epilepsiia i paroksizmalnye sostoianiia. 2019;11:395-406. doi: https://doi.org/10.17749/2077-8333.2019.11.4.395-406
- Gnezdickii VV, Korepina OS, Karlov VA, Novoselova GB. [Pathological signs of an epileptic and non-epileptic nature detected during wakefulness and during sleep during outpatient and ward monitoring of the EEG: problems of interpretation]. Epilepsiia i paroksizmalnye sostoianiia.2017;9:30-40. doi: https://doi.org/10.17749/2077-8333.2017.9.2.030-040
- Gromov SA, Sivakova NA. [Modern aspects of resolving epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2019;11:21-26. Russian. doi: https://doi.org/10.17749/2077-8333.2019.11.1.21-26
- Zavadenko NN, Nesterovskii JuE, Holin AA, Vorobeva IS. [Paroxysmal disorders in the long-term period of traumatic brain injury in children and adolescents]. Epilepsiia i paroksizmalnye sostoianiia. 2018;10:72-82. Russian. doi: https://doi.org/10.17749/2077-8333.2018.10.4.072-082
- Karlov VA. [The doctrine of the epileptic system. merit of the national scientific school]. Epilepsiia i paroksizmalnye sostoianiia. 2017;9:76-85. Russian. doi: https://doi.org/10.17749/2077-8333.2017.9.4.076-085
- Karlov VA, Inozemceva OS, Novoselova GB. [On the problem of sleep disorders in epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2017;9:36-39. Russian. doi: https://doi.org/10.17749/2077-8333.2017.9.1.036-039
- Kissin MYa, Seliutina EV. [Reasons for the development of nosogenic mental disorders in patients with epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2019;11:293-7. doi: https://doi.org/10.17749/2077-8333.2019.11.3.293-297
- Kitaeva VE, Kotov AS. [The effectiveness of treatment of patients with long-term course of medial temporal lobe epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2018;10:31-37. doi: https://doi.org/10.17749/2077-8333.2018.10.3.031-037
- Malyshev CM, Alekseeva TM, Hachatrian VA, Galagudza MM. [Pathogenesis of drug-resistant epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2019;11:79-87. Russian. doi: https://doi.org/10.17749/2077-8333.2019.11.1.79-87
- Odintsova GV, Aleksandrov MV, Ulitin AJu, Koloteva AV. [Influence of the duration of the disease on the course of epilepsy in neurosurgical patients]. Epilepsiia i paroksizmalnye sostoianiia. 2018;10:44-51. Russian. doi: https://doi.org/10.17749/2077-8333.2018.10.3.044-051
- Perepelova EM, Perepelov VA, Merkulova MS. [Some questions of MR diagnostics of microstructural and functional disorders of the brain in patients with temporal lobe epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2017;4:41-49.
- Rubleva JuV, Terian RA, Avakian GG, Savenkov AA, Solomatin JuV, Oleinikova OM, et al. [Clinical, neurophysiological and neuroimaging characteristics of adult patients with focal epilepsy with cardiac rhythm and conduction disturbances in the ictal period]. Epilepsiia i paroksizmalnye sostoianiia. 2018;10:14-24. Russian. doi: https://doi.org/10.17749/2077-8333.2018.10.1.014-024
- Sivakova NA, Korsakova EA, Lipatova LV. [Pathomorphosis of focal epilepsy and its neurophysiological correlates]. Epilepsiia i paroksizmalnye sostoianiia. 2018;10:6-13. doi: https://doi.org/10.17749/2077-8333.2018.10.1.006-013
- Sorokina ND, Percov SS, Selickii GV. [High-frequency bioelectric activity of the brain in the diagnosis of epilepsy]. Epilepsiia i paroksizmalnye sostoianiia. 2018;10:6-13. doi: https://doi.org/10.17749/2077-8333.2018.10.3.006-013
- Firsov KV, Kotov AS. [An observational study of the informativeness of routine EEG and nighttime EEG video monitoring in adult patients with epilepsy in real clinical practice]. Epilepsiia i paroksizmalnye sostoianiia. 2019;1:153-63.
- Amiri M, Hansen C. The interictal dysphoric disorder in patients with epilepsy: A doubtful disorder lacking diagnostic tools. Seizure. 2015;24:70-6. doi: https://doi.org/10.1016/j.seizure.2014.08.009
- Asadi-Pooya AA, Stewart GR, Abrams DJ, Sharan A. Prevalence and Incidence of Drug-Resistant Mesial Temporal Lobe Epilepsy in the United States. World Neurosurgery. 2017;99:662-6. doi: https://doi.org/10.1016/j.wneu.2016.12.074
- Brodie MJ. Practical Use of Newer Antiepileptic Drugs as Adjunctive Therapy in Focal Epilepsy. CNS Drugs.2015;29(11):893-904. doi: https://doi.org/10.1007/s40263-015-0285-4
- Chen Z, Brodie MJ, Liew D, Kwan P. Treatment outcomes in patients with newly diagnosed epilepsy treated with established and new antiepileptic drugs: A 30-year longitudinal cohort study. JAMA Neurology. 2018;75(3):279. doi: https://doi.org/10.1001/jamaneurol.2017.3949
- Ettinger AB, LoPresti A, Yang H, Williams B, Zhou S, Fain R, et al. Psychiatric and behavioral adverse events in randomized clinical studies of the noncompetitive AMPA receptor antagonist perampanel. Epilepsiia. 2015;56(8):1252-63. doi: https://doi.org/10.1111/epi.13054
- Kitaura H, Shirozu H, Masuda H, Fukuda M, Fujii Y, Kakita A. Pathophysiological Characteristics Associated With Epileptogenesis in Human Hippocampal Sclerosis. EBioMedicine. 2018;29:38-46. doi: https://doi.org/10.1016/j.ebiom.2018.02.013
- Mula M. The interictal dysphoric disorder of epilepsy: Legend or reality? Epilepsy Behavior. 2016;58:7-10. doi: https://doi.org/10.1016/j.yebeh.2016.02.018
- Noachtar S, Guldiken B. Diagnosis of non-epileptic paroxysmal disorders and epileptic seizures. 2017;88(10):1109-18. doi: https://doi.org/10.1007/s00115-017-0397-9
- Nobili L, Proserpio P, Combi R, Provini F, Plazzi G, Tassi L, et al. Nocturnal Frontal Lobe Epilepsy. Current Neurology and Neuroscience Reports. 2014;14(2). doi: https://doi.org/10.1007/s11910-013-0424-6
- Puttachary S, Sharma S, Stark S, Thippeswamy T. Seizure-Induced Oxidative Stress in Temporal Lobe Epilepsy. BioMed Research International. 2015;2015:1-20. doi: https://doi.org/10.1155/2015/745613
- Sone D, Matsuda H, Ota M, Maikusa N, Kimura Y, Sumida K, et al. Impaired cerebral blood flow networks in temporal lobe epilepsy with hippocampal sclerosis: A graph theoretical approach. Epilepsy Behav. 2016;62:239-45. doi: https://doi.org/10.1016/j.yebeh.2016.07.016
- Suda T, Tatsuzawa Y, Mogi T, Yoshino A. Interictal dysphoric disorder in patients with localization-related epilepsy: Diagnostic relationships with DSM-IV psychiatric disorders and the impact of psychosocial burden. Epilepsy Behavior. 2016;54:142-7. doi: https://doi.org/10.1016/j.yebeh.2015.11.020
Serdiuk V.M., Ustymenko S.B., Klopotska N.G., Tikhomirova V.V. Effect of orthokeratologic lenses of individual design on visual functions in premature babies with myopia in school age
Authors and affiliations
Serdiuk V.M. Dnipro State Medical University, Dnipro, Ukraine
Ustymenko S.B. Dnipro State Medical University; CE «Dnipropetrovsk Regional Clinical Ophthalmological Hospital», Dnipro, Ukraine
Key words: customized orthokeratological lenses, myopia, prematurely babies, school-age children
Abstract. Effect of orthokeratologic lenses of individual design on visual functions in premature babies with myopia in school age. Serdiuk V.M., Ustymenko S.B., Klopotska N.G., Tikhomirova V.V. The study included 62 children aged 6 to 14 years with mild to moderate myopia, born at 28-34 weeks of gestation with a birth weight of 970 to 2200 g. Selection criteria: corneal refractive power >46.00 D, no keratoconus or macular degeneration. The main group – 32 children (64 eyes), who were assigned orthokeratologic lenses, the control group – 30 children (60 eyes) – glasses users. Differences between the groups were not significant. Observation period was 3 years. There were no complications. Corrected visual acuity in the main group increased from 0.63±0.08 to 0.98±0.06, in the control – from 0.61±0.05 to 0.73±0.05; p<0.005. Stable restoration of binocular vision was observed in all children of the study group and 83% of children in the control one. Reserves of absolute accommodation in the main group during the observation period increased by 6.7±0.38 D, and in the control group – by 2.3±0.42 D (p<0.001), which is explained by the active use of accommodation by children of the main group. After 3 years of observation in the main group, the anteroposterior size of the eyeball, according to echobiometry data, almost did not change (from 22.32±0.9 to 24.02±1.1 mm, p>0.2), and in the control group, the eye elongation was more pronounced: from 22.45±0.8 to 25.94±0.9 mm (p<0.01). Complete stabilization of myopia was observed in 30 children (93.75%) of the study group. Orthokeratological lenses MoonLens can be used in patients with high corneal curvature; their use in prematurely babies with a high refractive power of the cornea (>46.0 D) allowed to obtain better results of the treatment.
- Lyajtman MV. [Examination in ophthalmology]. Moskva: GEOTAR-Media; 2016.
- Neroev VV, Katargina LA, Kogoleva LV. [The prevention of blindness and visual impairment in children with retinopathy of prematurity]. Voprosy sovremennoi pediatrii. 2015;14(2):265-70. Russian. doi: https://doi.org/10.15690/vsp.v14i2.1296
- Borisov DA, Saidasheva EI, Dautova ZA, et al. [Оcular surface in long terms of ortokeratology lenses’s use in adolescents with myopia]. Oftalmolohiia. 2020;17(2):223-8. Russian. doi: https://doi.org/10.18008/1816-5095-2020-2-223-228
- Efron N, Pritchard N, Brandon K, et al. A survey of the use of grading scales for contact lens complications in optometric practice. Clinical and Experimental Optometry. 2011;94(2):193-9. doi: https://doi.org/10.1111/j.1444-0938.2010.00549.x
- Zha Y, Zhu G, Zhuang J, et al. Axial length and ocular development of premature infants without ROP. Journal of ophthalmology. 2017. ID 6823965. doi: https://doi.org/10.1155/2017/6823965
- Lee YC, Wang JH, Chiu CJ Effect of orthokeratology on myopia progression: twelve-year results of a retrospective cohort study. BMC ophthalmology. 2017:17(1):1-8. doi: https://doi.org/10.1186/s12886-017-0639-4
- Kang P, Watt K, Chau T, et al. The impact of orthokeratology lens wear on binocular vision and accommodation: A short-term prospective study. Contact Lens and Anterior Eye. 2018;41(6):501-6. doi: https://doi.org/10.1016/j.clae.2018.08.002
- Gifford KL, Gifford P, Hendicott L, et al. Zone of clear single binocular vision in myopic orthokeratology. Eye & contact lens. 2020;46(2):82-90. doi: https://doi.org/10.1097/ICL.0000000000000614
Garibeh E., Bondаr S.А., Tokarchuk N.I., Vyzgha Y.V. Charactirestics of vitamin D level in patients with atopic dermatitis
Authors and affiliations
Key words: atopic dermatitis, vitamin D
Abstract. Charactirestics of vitamin D level in patients with atopic dermatitis. Garibeh E., Bondаr S.А., Tokarchuk N.I., Vyzgha Y.V. The peculiarity of the skin is that it acts not only as a place of synthesis of vitamin D, but also as an organ targeted for its biologically active form. The aim of our study was to analyze the level of vitamin D in patients with atopic dermatitis. There were examined 48 people aged between 18 to 55 years; they are residents of Vinnytsia and Vinnytsia region. Serum levels of vitamin D, total IgE and eosinophilic cationic protein (ECP) were determined in the subjects. The average level of vitamin D in the serum of patients with atopic dermatitis was 19.2 [11.3-25.4] ng/ml, which corresponded to a deficiency. Among those surveyed, vitamin D deficiency was found in 68.4±4.7% (n=26) while vitamin D insufficiency in 31.6±4.5% (n=12). The severe course of the disease prevailed among patients aged 18-40 years (63.33±8.79) % more than in the age group of 41 years and older, (36.67±8.8%, p<0.05; OR=2.98, S=0.53, 95% SI:1.04-8.52). The proportion of people with vitamin D deficiency and moderate severity of atopic dermatitis was 62.5% (n=10) with a median level of 14 [8.3–19] ng/ml, and patients with severe atopic dermatitis made up 90.9% (n=20) (χ²=4.6; p=0.023), in which the median level of vitamin D was 14 [8.3-19] ng/ml. Serum vitamin D levels were in the zone of deficiency in patients with moderate and severe atopic dermatitis. Vitamin D deficiency was significantly more common in the group of patients with elevated levels of allergic inflammation markers. A positive correlation of medium strength between the level of vitamin D and ECP in the serum of patients with atopic dermatitis (rs=0.53, p<0.001), was revealed.
- Kang JW, Kim JH, Kim HJ, et al. Association of serum 25-hydroxyvitamin D with serum IgE levels in Korean adults. Auris, Nasus, Larynx. 2015;43(1):84-88. doi: https://doi.org/1016/j.anl.2015.06.010
- Bergqvist C, Ezzedine K. Vitamin D and the skin: what should a dermatologist know? G Ital Dermatol Venereol.2019;154(6):669-80. doi: https://doi.org/10.23736/S0392-0488.19.06433-2
- Ferreira CES, Maeda SS, Batista MC, Lazaretti-Castro M, Vasconcellos LS, Madeira M, et al. Consensus – reference ranges of vitamin D [25(OH) D] from the Brazilian medical societies Brazilian Society of Clinical Pathology/Laboratory Medicine (SBPC/ML) and Brazilian Society of Endocrinology and Metabolism (SBEM). Jurnal Brasileiro de Patologia e Medicina Laboratorial. 2017;53(6):377-81. doi: http://www.dx.doi.org/10.5935/1676-2444.20170060
- Galvão AA, Sena FA, Belitardo EM, Santana MB, Costa GN, Cruz BA, et al. Genetic polymorphisms in vitamin D pathway influence 25(OH)D levels and are associated with atopy and asthma. Allergy Asthma Clin Immunol. 2020;16:62-75. doi: https://doi.org/10.1186/s13223-020-00460-y
- Han YY, Forno E, Celedón JC. Vitamin D insufficiency and asthma in a U.S. Nationwide study. J Allergy Clin Immunol Pract. 2017;5(3):790-796. doi: https://doi.org/10.1016/j.jaip.2016.10.013
- Hemanth KK, Sathisha Upparahalli V, Anil M. Synergy of Interleukin (IL)-5 and IL-18 in eosinophil mediated pathogenesis of allergic diseases. Cytokine Growth Factor Rev. 2019;47:83-98. doi: https://doi.org/10.1016/j.cytogfr.2019.05.003
- ThorisdottirD, Gunnarsdottir I, Vidarsdottir AG, Sigurdardottir S, Bryndis EB, Thorsdottir Infant Feeding, Vitamin D and IgE Sensitization to Food Allergens at 6 Years in a Longitudinal Icelandic Cohort. Nutrients. 2019;11(7):1690. doi: https://doi.org/10.3390/nu11071690
- AmberKT, Chernyavsky A, Agnoletti AF, Cozzani E, Grando Mechanisms of pathogenic effects of eosinophil cationic protein and eosinophil‐derived neurotoxin on human keratinocytes. Experimental Dermatology. 2018;27(12):1322-7. doi: https://doi.org/10.1111/exd.13782
- Navarro-Triviño FJ, Arias-Santiago S, Gilaberte-Calzada Y. Vitamin D and the Skin: A Review for Dermatologists. Actas Dermosifiliogr. 2019;110(4):262-72. doi: https://doi.org/10.1016/j.ad.2019.04.001
- Petrie A, Sabin C. Medical Statistics at a Glance. 4th edition. Wiley-Blackwell; 2019. ISBN: 978-1-119-16783-9.
- Cheon BR, Shin JE, Kim YJ, Shim JW, Kim DS, Jung HL, Park MS, Shim JY. Relationship between serum 25-hydroxyvitamin D and interleukin-31 levels, and the severity of atopic dermatitis in children. Korean J Pediatr. 2015; 58(3): 96-101. doi: https://doi.org/3345/kjp.2015.58.3.96
- AmonU, Baier L, Yaguboglu R, Ennis M, Holick MF, Amon Serum 25-hydroxy vitamin D levels in patients with skin diseases including psoriasis, infections, and atopic dermatitis. Dermato-Endocrinology. 2018;10(1):e1442159-14. doi: https://doi.org/10.1080/19381980.2018.1442159
- Zhang L, Zhang S, He C, Wang X. VDR Gene Polymorphisms and Allergic Diseases: Evidence from a Meta-analysis. Immunol Invest. 2020;49(1-2):166-77. doi: https://doi.org/10.1080/08820139.2019.1674325
- Umar M, Sastry KS, Ali FA, Al-Khulaifi M, Wang E, Chouchane AI. Vitamin D and the Pathophysiology of Inflammatory Skin Diseases. Skin Pharmacology and Physiology. 2018;31(2):74-86. doi: https://doi.org/10.1159/000485132
- Yuan K, Madu CO, Lu Y. Immunological Role of Vitamin D in Skin Diseases and Carcinoma. Oncomedicine. 2017;2:52-60. doi: https://doi.org/10.7150/oncm.19262
Badiul P.O., Korpusenko O.I., Nor N.M., Korpusenko I.V., Rudenko O.I. Influence of negative pressure on changes in blood supply in donor zones of perforant flaps formation
Authors and affiliations
Badiul P.O. Dnipro State Medical University, Dnipro, Ukraine,
Korpusenko O.I. Dnipro State Medical University, Dnipro, Ukraine,
Nor N.M. Dnipro State Medical University, Dnipro, Ukraine,
Korpusenko I.V. Dnipro State Medical University, Dnipro, Ukraine,
Key words: reconstructive surgery, negative pressure, perforating flaps, donor sites, dynamic thermography, blood supply
Abstract. Influence of negative pressure on changes in blood supply in donor zones of perforant flaps formation. Badiul P.O., Korpusenko O.I., Nor N.M., Korpusenko I.V., Rudenko O.I. Our study is based on the establishment of normative indicators of the effect of negative pressure on the skin of the thigh. To do this, a group of healthy volunteers was examined – 35 people: 17 women and 18 men aged 19 to 58. The study was conducted on the clinical basis of the burn centre in 2020-2021. The local vacuum was created using a negative pressure device “AGAT-Dnepr” by applying a silver-containing polyurethane sponge directly on the front surface of the thigh. The pressure (P) – 110-160 mmHg has been applied for 30 minutes. Tissue perfusion was monitored by laser Doppler flowmetry and thermal imaging research. Measurements were performed before vacuum, immediately after the end of the application of negative pressure, for three days at the same time at a constant temperature. According to the results of the cluster analysis, based on the effect of negative pressure on the thigh skin, patients with three types of reactions were identified – low, medium and high perfusion changes. The perfusion level of tissue volume per unit time increased the most rapidly: in the group of high perfusion by 45.56 (95% CI 42.09-51.96) PU; in the group of average perfusion – by 22.49 (95% CI 19.93-28.99) PU; in the low perfusion group – by 22.43 (95% CI 15.90-25.13) PU. The number of erythrocytes per unit tissue changed at a medium rate: in the high perfusion group by 57.77 (95% CI 53.05-62.48) AU; in the group of average perfusion – by 55.86 (95% CI 49.62-65.06) PU; in the low perfusion group – by 34.85 (95% CI 13.19-53.55) PU. Among those surveyed, 12 people were included in the low perfusion group (34.29%), 16 people in the medium perfusion group (45.71%) and 7 people in the high perfusion group (20.0%). Before VAC-action the ascending level of thigh skin temperature T1 ranged from 28.60 to 32.90°C and averaged 31.46 (95% CI 30.97-31.95)°C. Immediately, after the offset of the negative pressure the area of the fields of local skin temperature rise increased, the average temperature level ranged from 30.60 to 35.0°C and the average T2 was 33.59 (95% CI 33.16-34.01)°C. The temperature rise occurred on average by 2.13 (95% 1.72-2.54)°C, which corresponded to an increase of 6.77% with significant (p<0.001) statistical differences. Only 1 examined had a slight decrease in the temperature of the thigh skin from 31.9 to 31.6°C, in the rest of the examined the rise was by 6.4°С maximally, from 28.6 to 35.0°С, there was a temperature rise by 2°С or more degrees among 9 people (25.71%). After 24 hours there was a rise in local body temperature among all examined (100%), after 48 hours – among 25 (71.43%), and after 72 hours – among 21 examined (60.0%). When choosing a donor site of the perforant flaps, we recommend relying on areas which will belong to the group of indicators with high perfusion. According to the analysis, the group of high perfusion will include patients with an initial average level of perfusion in the donor areas per unit time of 15.84 (14.47-17.21) PU, the number of erythrocytes per unit tissue – 58.73 (53.44-64.02) AU and skin temperature – 29.23 (28.65-29.80)°С; to the average according to F rates – 14.36 (13.0-15.73) PU, C rates – 45.37 (39.74-51.0) AU, T rates – 28.17 (27.74-28.60)°С; to the group of low perfusion, respectively, according to F rates – 12.38 (10.74-14.0) PU, C rates – 43.89 (40.18-47.61) AU, T rates – 29.79 (29.17-30.40)°С. In general, the effect of negative pressure contributes to increased perfusion and local microcirculation in tissues, as evidenced by dynamic infrared thermography: increased “hot” skin fields, increased temperature with a temperature gradient ∆T2.1=2.13 (95% 1.72-2.54)°C and for indicators of laser Doppler flowmetry – increase in perfusion by 2.42 times (p<0.001).
- Sliesarenko SV, Badiul PO. [Perforator flaps in reconstructive surgery]. Atlas. Dnipro: ART-PRESS; 2021. Ukrainian.
- Paik JM, Pyon JK. Risk Factor Analysis of Freestyle Propeller Flaps. J Reconstr Microsurg. 2017;33:26-31. doi: https://doi.org/10.1055/s-0036-1586748
- Sliesarenko SV, Badiul PO, Hong JP, Sliesarenko KS, Korpusenko EI. [Basic principles and modern reconstruction algorithm for combined injuries of the lower extremities]. Plastic, reconstructive and aesthetic surgery. 2017;(3-4):49-74. Ukrainian. doi: https://doi.org/10.1097/GOX.0000000000000736
- Hong Joon Pio, Changsik John Park, Hyunsuk Peter Suh. Importance of Vascularity and Selecting the Recipient Vessels of Lower Extremity Reconstruction. Journal of Reconstructive Microsurgery. [Internet]. 2021:72-74. [cited 2021 Oct 09]. doi: https://doi.org/10.1055/b-0041-179911
- Sliesarenko SV, Badiul PO, Korpusenko EI, Nor NM, inventors. [Method of preoperative preparation of donor zones for plastics of defects of covering tissues]. Ukrainian patent 143213, UA, IPC А61В 5/01 (UA). 2020 July 10.
- Della Croce FJ, Sullivan SK, Talavera Fr, Long James Neal. Perforator Flap Breast Reconstruction Treatment & Management. [Internet]. 2019 [cited 2021 Oct 09]. Available from: https://www.medscape.com/answers/1276406-164050/what-are-the-possible-complications-of-perforator-flap-breast-reconstruction#qna
- Koshima I. [Perforator flaps concept, history and evolution of the use of anterolateral thigh flap (ALT)]. Reconstructive and plastic surgery issues. 2020;73(2):5-12. Russian.
- Badiul PO, Sliesarenko SV. [Reconstruction of extensive soft tissue defects using an anterolateral thigh perforator flap]. Surgery of Ukraine. 2017;(3):12-19. Ukrainian.
- Badiul PA, Sliesarenko SV, Rudenko ОI. [Influence of negative pressure on the state of interperforating anastomoses and preoperative preparation of the donor zone of perforating flaps]. Plastic, reconstructive and aesthetic surgery. Abstracts of the 5th VAPREH Congress. Kyiv; 2019. Ukrainian.
- Badiul P, Sliesarenko S, Rudenko O. [Cutaneous Negative Pressure Application as the method of improving microcirculation in the donor site of the perforator flap]. Chirurgia plastyczna i oparzenia. 2019;7(3):115. Ukrainian.
- Badiul PO, Sliesarenko SV, Saliaiev MG, Kriachkova LV. [Hypnosis Influence on the Perfusion in Perforator Flaps in Early Postoperative Period: A Series of 18 Cases]. Plastic and Reconstructive Surgery. Global Open. 2019;11(7):e2491. Ukrainian. doi: https://doi.org/10.1097/GOX.0000000000002491
- Yin Y, Zhang R, Li S, Guo J, Hou Z, Zhang Y. Negative-pressure therapy versus conventional therapy on split-thickness skin graft: A systematic review and meta-analysis. Int. Surg. 2018;50:43-48. doi: https://doi.org/10.1016/j.ijsu.2017.12.020
- Badiul P, Sliesarenko S, Cherednichenko N, Rudenko O. CT angiography and dynamic thermography in the perforator flaps preplanning. The 4-th Congress of APFSRM and WSRM Symposium. Turkey: Antalya. 2018 May 09-13. Ukrainian.
- Cherednichenko NА, Badiul PА, Sliesarenko SV, Kulikova FI, Rudenko ОI. [Dynamic infrared thermography in planning lower limb reconstructive surgery]. Radiation diagnostics, radiation therapy. 2017;4:54-58. Ukrainian.
- Badiul PO, Sliesarenko SV, Cherednychenko NO, Morgun OV. Efficiency Assessment of Multidetector-Row Computed Tomographic Angiography Using Reconstruction With Locoregional Perforator Flaps. Plastic Surgery.2021 June. doi: https://doi.org/10.1177/22925503211024750
- Badiul PO, Samoilenko GE, Nor NM, Sliesarenko KS. [Possibilities of application of vacuum therapy at disturbances of venous outflow in islet flaps]. Clinical surgery. 2015;7:52-54. Ukrainian.
- Taylor GI, Corlett RJ, Dhar SC, Ashton MW. The anatomical (angiosome) and clinical territories of cutaneous perforating arteries: Development of the concept and designing safe flaps. Plastic and Reconstructive Surgery. 2011;127(4):1447-59. doi: https://doi.org/10.1097/PRS.0b013e318208d21b
- Tenekeci G. Perforator Flaps: Principles and Techniques. [Internet]. 2017 [cited 2021 Oct 09]. doi: https://doi.org/10.5772/intechopen.71270
Mahdi Afshari, Iraj Shahramian, Morteza Salarzaei, Hadi Mirzaie, Fateme Parooie. Opium use and COVID-19 infection, a retrospective study in Iran
Authors and affiliations
Mahdi Afshari Zabol University of Medical Sciences, Pediatric Gastroenterology and Hepatology Research Center, Zabol, Iran
Iraj Shahramian Zabol University of Medical Sciences, Pediatric Gastroenterology and Hepatology Research Center, Zabol, Iran
Morteza Salarzaei Zabol University of Medical Sciences, Pediatric Gastroenterology and Hepatology Research Center, Zabol, Iran
Hadi Mirzaie Zabol University of Medical Sciences, Pediatric Gastroenterology and Hepatology Research Center, Zabol, Iran
Key words: opium, addiction, Covid-19
Abstract. Opium use and COVID-19 infection, a retrospective study in Iran. Mahdi Afshari, Iraj Shahramian, Morteza Salarzaei, Hadi Mirzaie, Fateme Parooie. The situation of Covid-19 patients with opium consumption has not been comprehensively investigated especially in Iran. This study is aimed to investigate the effect of current opium addiction on the severity and outcome of the Covid-19 infection. During a retrospective cohort study, clinical, demographic and all variables related to the severity and death due to the Corona virus infection were collected from the medical records of patients hospitalized from February 2020 to June 2021 in the study area. The role of current addiction in the severity and outcome of the disease was investigated using Cox, logistic and linear regression models. Of 939 confirmed Covid-19 cases admitted during the study period, 70 (7.5%) patients were currently addicted. Multivariate regression models showed that current opium addiction significantly increased the chance of endotracheal intubation (OR=2.96, p=0.005) and reduced the mean length of stay in hospital (beta coefficient =-0.42, p<0.0001). However, opium addiction did not change the risk of death (HR= 1.54, p=0.063) or O2 saturation (OR=1, p>0.99). Opium addiction can be associated with the severity of Covid-19 infection, but does not play a considerable role in the mortality of the patients.
- Lai CC, Shih TP, Ko WC, Tang HJ, Hsueh PR. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19): The epidemic and the challenges. Int J Antimicrobe Agents. 2020;55(3):105924. doi: https://doi.org/10.1016/j.ijantimicag.2020.105924
- Farhoudian A, Baldacchino A, Clark N, Gerra G, Ekhtiari H, Dom G, et al. COVID-19 and substance use disorders: Recommendations to a comprehensive healthcare response. An International Society of Addiction Medicine Practice and Policy Interest Group Position Paper. BasicClin 2020;11(2):133-50. doi: https://doi.org/10.32598/bcn.11.covid19.1
- Saeedi M, Omrani-Nava V, Maleki I, Hedayatizadeh-Omran A, Ahmadi A, Moosazadeh M, et al. Opium Addiction and COVID-19: Truth or false beliefs. Iran J Psychiatry Behav Sci. 2020;14(2):e103509. doi: https://doi.org/10.5812/ijpbs.103509
- Irwin A, Valentine N, Brown C, Loewenson R, Solar O, Brown H, et al. The commission on social determinants of health: Tackling the social roots of health inequities. PloS 2006;3(6):e106. doi: https://doi.org/10.1371/journal.pmed.0030106
- Babaei HA, Ramezankhani A, Barekati H, Vejdani M, Shariatinejad K, Panahi R, et al. Prevalence of substance abuse among dormitory students of Shahid Beheshti University of Medical Sciences, Tehran, Iran. Int J High Risk Behav Addict. 2015;4(2):e22350. doi: https://doi.org/10.5812/ijhrba.22350v2
- Aflatoonian MR, Sharifi I, Aflatoonian B, Divsalar K. Changes in the pattern of opium addiction in Bam after the earthquake. Report of Health Care. 2015;1(3):104-8.
- Ziaee M, Hajizadeh R, Khorrami A, Sepehrvand N, Momtaz S, Ghaffari S. Cardiovascular complications of chronic opium consumption: A narrative review article. Iran J Public Health. 2019;48(12):2154-64. doi: https://doi.org/10.18502/ijph.v48i12.3546
- Dubey S, Biswas P, Ghosh R, Chatterjee S, Dubey MJ, Chatterjee S, et al. Psychosocial impact of COVID-19. Diabetes Metab Syndr. 2020;14(5):779-88. doi: https://doi.org/10.1016/j.dsx.2020.05.035
- Nabati S, Asadikaram G, Arababadi MK, Shahabinejad G, Rezaeian M, Mahmoodi M, et al. The plasma levels of the cytokines in opium-addicts and the effects of opium on the cytokines secretion by their lymphocytes. Immunol 2013;152(1):42-6. doi: https://doi.org/10.1016/j.imlet.2013.04.003
- Pirnia B, Dezhakam H, Pirnia K, Malekanmehr P, Soleimani AA, Zahiroddin A, et al. COVID-19 pandemic and addiction: Current problems in Iran. Asian J Psychiatr. 2020;54:102313. doi: https://doi.org/10.1016/j.ajp.2020.102313
- Farhoudian A, Baldacchino A, Clark N, Gerra G, Ekhtiari H, Dom G, et al. COVID-19 and SubstanceUse Disorders: Recommendations to a Comprehensive Healthcare Response. An International Society of Addiction Medicine Practice and Policy Interest Group Position Paper. Basic Clin Neurosci. 2020;11(2):133-50. doi: https://doi.org/10.32598/bcn.11.covid19.1
- Wang QQ, Kaelber DC, Xu R, Volkow ND. COVID-19 risk and outcomes in patients with substance use disorders: analyses from electronic health records in the United States. Molecular psychiatry. 2021 Jan;26(1):30-9. doi: https://doi.org/10.1038/s41380-020-00880-7
- Dolati-Somarin A, Bahareh AN. The Reasons for Higher Mortality Rate in Opium Addicted Patients with COVID-19: A Narrative Review. Iranian Journal of Public Health. 2021 Mar;50(3):470. doi: https://doi.org/10.18502/ijph.v50i3.5587
- Volkow ND. Collision of the COVID-19 and Addiction Epidemics. Ann Intern Med. 2020;173(1):61-62. doi: https://doi.org/10.7326/M20-1212
Samoilenko A.V., Titovska S.O. Matrix metalloproteinases in the oral fluid as a characteristic of inflammatory-destructive process in periodontal tissues
Key words: generalized periodontitis, pathogenesis, diagnosis, matrix metalloproteinases
Abstract. Matrix metalloproteinases in the oral fluid as a characteristic of inflammatory-destructive process in periodontal tissues. Samoilenko A.V., Titovska S.O. The study of the imbalance between the activity of matrix metalloproteinases and their regulation markers in the oral fluid of patients with generalized periodontitis is necessary for creation of effective methods for early diagnosis and preventive treatment of the disease. The aim of the research was to examine the oral fluid of persons with healthy periodontal tissues, patients with chronic diffuse gingivitis and generalized periodontitis for concentrations of matrix metalloproteinases and their inhibitor, anti- and pro-inflammatory cytokines, markers of bone metabolism and to compare obtained results. 90 persons aged 32-45 years were examined, including 30 persons with intact periodontal tissues, 30 patients with chronic diffuse catarrhal gingivitis and generalized periodontitis of the initial degree and 30 ones with chronic generalized periodontitis of I-II degrees. Periodontal status was assessed using clinical indices and bone mineral density according to computed tomography data. The method of enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration of matrix metalloproteinases MMP-2, MMP-3, MMP-8, MMP-9, MMP-13, tissue inhibitor of metalloproteinases TIMP-1, interleukins-1β, -6 and -4, bone metabolism markers – tartrate-resistant acidic phosphatase and bone alkaline phosphatase, osteocalcin in the oral fluid. The initiation of the inflammatory-destructive process in the periodontal tissues was characterized by an increase levels of MMP and pro-inflammatory IL-1β and IL-6 (р<0.05), a decrease in the concentration of TIMP-1 (р>0.05) and anti-inflammatory IL-4 (р<0.05), against the background of the absence of dynamics of bone metabolism markers in the oral fluid. The progression of the process at the І-II degrees of periodontitis was accompanied by a further increase in the concentration of pro-inflammatory interleukins, an increase in the activity of tartrate-resistant acid phosphatase and a decrease in bone alkaline phosphatase and osteocalcin (р<0.05) in the oral fluid, while the content of MMP did not change (р>0.05). The expression of MMP is important for the preventive diagnosis of generalized periodontitis, but it is not indicative for determining the severity of the disease. The imbalance of MMP and TIMP-1 in the early stages of the pathological process indicates the greatest expediency of using MMP inhibitors in the treatment of patients with gingivitis and periodontitis of the initial degree.
- Antomonov MY. [Mathematical processing and analysis of biomedical data]. Kyiv; 2017. Russian.
- Vozna IV, Samoilenko AV, Pavlov SV. [Study of the content of biochemical markers of bone metabolism in the oral fluid of patients with generalized periodontitis]. Ukrainskyi stomatolohichnyi almanakh. 2020;4:10-14. doi: https://doi.org/10.31718/2409-0255.4.2020.02
- Danilevsky MF, Borisenko AV, Antonenko MY. [Therapeutic dentistry]. Kyiv: VSV «Medytsyna»; 2018. Ukrainian.
- Fastovets OO, Lucash AY. [Matrix metalloproteinase-8 in the early diagnosis of generalized periodontitis]. Zaporozhye Medical Journal; 2018;5(110):723-8.
- Abusleme L, Hoare A, Hong BY, Diaz PI. Microbial signatures of health, gingivitis, and periodontitis. Periodontol 2021;86(1):57-78. doi: https://doi.org/10.1111/prd.12362
- Baddam H, Vivekanandan G, Kondreddy K, Peddi S, Chitnis PP, Singh YP, et al. Evaluation of gingival crevicular fluid and serum tartrate-resistant acid phosphatase levels in subjects with clinically healthy periodontium and chronic periodontitis: A clinical-biochemical study. J Pharm Bioallied Sci. 2021;13(Suppl 2):1275-9. doi: https://doi.org/10.4103/jpbs.jpbs_90_21
- Bostanci N, Mitsakakis K, Afacan B, Bao K, Johannsen B, Baumgartner D, et al. Validation and verification of predictive salivary biomarkers for oral health. Sci Rep.2021;11(1):6406. doi: https://doi.org/10.1038/s41598-021-85120-w
- Cafiero C, Spagnuolo G, Marenzi G, Martuscelli R, Colamaio M, Leuci S. Predictive periodontitis: the most promising salivary biomarkers for early diagnosis of periodontitis. JClin 2021;10(7):1488. doi: https://doi.org/10.3390/jcm10071488
- de Brouwer P, Bikker FJ, Brand HS, Kaman WE. Is TIMP-1 a biomarker for periodontal disease? A systematic review and meta-analysis. J Periodontal Res. 2021;57(2):235-45. doi: https://doi.org/10.1111/jre.12957
- Deng K, Pelekos G, Jin L, Tonetti MS. Diagnostic accuracy of a point-of-care aMMP-8 test in the discrimination of periodontal health and disease. J Clin Periodontol.2021;48:1051-65. doi: https://doi.org/10.1111/jcpe.13485
- Elashiry M, Morandini AC, Cornelius Timothius CJ, Ghaly M, Cutler CW. Selective antimicrobial therapies for periodontitis: win the “battle and the war”. Int. Mol. Sci. 2021;22:6459. doi: https://doi.org/10.3390/ijms22126459
- Fastovets OO, Masheiko IV, Lucash AY. Evaluation of bone resorptive potential in the treatment of generalized periodontitis. Wiad Lek. 2020;73(11):2396-402. doi: https://doi.org/36740/WLek202011112
- Pan W, Wang Q, Chen Q. The cytokine network involved in the host immune response to periodontitis. Int JOral 2019;11:30. doi: https://doi.org/10.1038/s41368-019-0064-z
- Virtanen E, Yakob M, Tervahartiala T, Söder PÖ, Andersson LC, Sorsa T, et al. Salivary MMP-13 gender differences in periodontitis: A cross-sectional study from Sweden. Clin Exp Dent Res. 2017;3(5):165-70. doi: https://doi.org/10.1002/cre2.76
Polka N.S., Makhniuk V.M., Chorna V.V., Podolian V.M., Yurchenko S.T. Hygienic assessment of new architectural and planning solutions of buildings of psychiatric health care facilities
Authors and affiliations
Key words: psychiatric hospitals, design decisions, multidisciplinary approach, healthcare environment
Abstract. Hygienic assessment of new architectural and planning solutions of buildings of psychiatric health care facilities. Polka N.S., Makhniuk V.M., Chorna V.V., Podolian V.M., Yurchenko S.T. Aim: to conduct a hygienic assessment of new architectural and planning solutions of psychiatric health care facility buildings in Ukraine and give a comparative description of the sanitary standards of the latter in the EU. The study was conducted based on the analysis of State Building Norms (SBN) B.2.2-10:2019 “Buildings and structures. Health care facilities” (draft, final revision), scientific sources of domestic and foreign scientists. A survey of the chief physicians of psychiatric hospitals of Ukraine concerning safe sanitary and hygienic, anti-epidemic conditions for the functioning of psychiatric health care facilities was conducted. We consider it necessary to present more carefully architectural and planning solutions in the relevant section of SBN B.2.2-10:2019 (draft, final revision) or to use the opportunity specified in the introduction to these Norms, and to supplement them with the Manual on the design of psychiatric health care facilities, taking into account the experience of the European Union. To create an appropriate “therapeutic environment” in new domestic mental health care facilities, it is necessary to implement European requirements in the design of these facilities with the involvement of multidisciplinary groups: (from architects to nurses, from construction contractors to patients) and supplement SBN B.2.2-10:2019 “Buildings and structures. Health care Facilities” of Ukraine with a guide for designing facilities of a new type of “Mental Health Centers” as in the Republic of Poland. We have developed and sent to the chief physicians of psychiatric hospitals of Ukraine «Questionnaire for scientific sanitary and epidemiological assessment of the conditions of placement of a psychiatric health care facility”. Based on the analysis of the block of questions on sanitary and antiepidemic and sanitary and hygienic parameters that characterize the buildings of domestic psychiatric hospitals, the location of medical structures and auxiliary units on the land plot, i.e. the design of buildings is determined. In particular, 50% of psychiatric hospitals are housed in combined buildings, 25% have a pavilion system (separate buildings), 12.5% each have a centralized system (all in one building) and a block system. The number of stories of psychiatric hospitals up to 2 is 50%, up to 3 – 37.5% and up to 5 stories in those which have been under construction since 1960 – 12.5%. Analysis of the questionnaire block on the conditions of stay, treatment, rehabilitation of the mentally ill revealed the possibility of organizing occupational therapy in 12.5% of psychiatric hospitals, where special workshops are equipped and patients can acquire professional skills. Physiotherapy rooms are equipped in 50% of psychiatric hospitals. Low provision of patients with furniture was revealed: 25% of patients partially have proper desk, 75% do not have it; 50% are provided with proper chairs; 62.8% use proper bedside tables and 25% – closets for storing personal clothes.
- [Conceptual note. VPA Expert Committee on the Mental Health Crisis in Ukraine]. World Psychiatric Association. Advance Psychiatry and Mental Health Across the World. [Internet]; 2020 June. Ukrainian. Available from: https://www.gip-global.org/files/ukraine-policy-brief-final-ua.pdf
- Chorna VV. [Determination of mental health of the population of Ukraine and EU countries]. Environment and 2020;2(95):47-53. Ukrainian. doi: https://doi.org/10.32402/dovkil2020.02.047
- [State building norms of Ukraine «Buildings and constructions of Health care institutions» project (first edition) SBN B.2.2-10:2019]. Ukrainian. Available from: https://www.minregion.gov.ua/wp-content/uploads/2019/08/Persha-redaktsiya-DBN-Ohorona-zdorovya.pdf
- Chorna VV. [Motivation and efficiency of mental health care workers as a predictor of their psychological well-being]. Environment and health. 2020;4(97):53-62. Ukrainian. doi: https://doi.org/10.32402/dovkil2020.04.053
- Carr RF. Psychiatric Facility NIKA. for VA Office of Construction & Facilities Management (CFM). 2017. Availablefrom:
- Design Guidance for Psychiatric Intensive Care Units. Low Secure Units (NAPICU) Design in Mental HealthNetwork; Available from: https://napicu.org.uk/wp-content/uploads/2017/05/Design-Guidance-for-Psychiatric-Intensive-Care-Units-2017.pdf
- Ulrich RS, Bogren L, Gardiner S. Psychiatric ward design can reduce aggressive behavior. Journal of Environmental 2018;57:53-66. doi: https://doi.org/10.1016/j.jenvp.2018.05.002
- Hunt J, Sine D. Facility Guidelines Institute Design Guide for the Built Environment of Behavioral Health Facilities.Edition 7.0; Available from: https://www.fgiguidelines.org/wp-content/uploads/2017/03/DesignGuideBH _7.2_1703.pdf
- Hsu T, Ryherd E, Waye K, Ackerman J. Noise pollution in hospitals: Impact on patients. Jornal of Clinical Outcomes Management. 2012;19(7):301-9.
- Jue K, Nathan-Roberts D. How noise affects patients in hospital. Sage Journal. 2019;63(1):1510-4. doi: https://doi.org/10.1177/1071181319631325
- Papoulias C, Csipke E, Rose D, Wykes T. The psychiatric ward as a therapeutic space: systematic review. The British Journal of Psychiatry. 2014;205(3):171-6. doi: https://doi.org/10.1192/bjp.bp.114.144873
- Coburn A, Kardan O, Kotabe H, Berman G. Psychological responses to natural patterns in architecture. Journal of Environmental Psychology. 2019;62:133-45. doi: https://doi.org/10.1016/j.jenvp.2019.02.007
- International Health Facility Guidelines. Part B-Health Facility Briefing & Design 200 Mental Health Unit – Oider Persons; 2014. Available from: https://healthfacilityguidelines.com/ViewPDF/ViewIndexPDF/iHFG_part_b_complete
- Kovalev YM, Kfia DV. [Color solution of interiors of psychiatric hospitals]. Theory and practice of design. 2013;3:58-59. Ukrainian. Available from: http://nbuv.gov.ua/UJRN/
- Mental health in transition: evaluation results and recommendations for the integration of mental health into the primary care system and community platforms in Ukraine. International Medical Corps with the support of the World Bank Group. World Bank Group. Available from: https://www.worldbank.org/
Dorohan` S.B., Shevchenko A.A., Kulagin A.A., Liashchenko O.V., Lobas V.M., Mikriukova N.G., Kostetsky I.V. Perception of the epidemic risks of the COVID-19 pandemic by the population of Ukraine
Authors and affiliations
Dorohan` S.B. Donetsk National Medical University, Kropyvnytskyi, Kirovohrad region, Ukraine
Kulagin A.A. Dnipro State Medical University, Dnipro, Ukraine
Liashchenko O.V. Dnipro State Medical University, Dnipro, Ukraine
Lobas V.M. Donetsk National Medical University, Kropyvnytskyi, Kirovohrad region, Ukraine
Mikriukova N.G. Donetsk National Medical University, Kropyvnytskyi, Kirovohrad region, Ukraine
Key words: coronavirus infection, epidemic risks, COVID-19, population vigilance, scale of epidemiological vigilance
Abstract. Perception of the epidemic risks of the COVID-19 pandemic by the population of Ukraine. Dorohan` S.B., Shevchenko A.A., Kulagin A.A., Liashchenko O.V., Lobas V.M., Mikriukova N.G., Kostetsky I.V. In the social network Facebook from April 2020 to October 2020 an online survey using the "Google Form" was conducted to study the perception of the COVID-19 pandemic by the residents of Ukraine. The study involved 550 participants residing in the Ukraine-controlled territory and the city of Kyiv. The data are as follows: the coronavirus is considered a serious danger by 33.3±2.0% of respondents and 11.6±1.4% agree with certain doubts (in total – Σ44.9±2.1%); 21.1±1.7% believe that the coronavirus is not serious and 17.3±1.6% believe that it is probably not serious (Σ38,4±2.1%). COVID-19 is recognized as a pandemic by 73.6±1.9% of respondents, of them 45.6±2.1% of respondents completely agree with this statement. Different views towards the severity of the problem show little but reliable correlation with age (rc =0,23; p<0.001), gender (rc =0,21; p<0,001) of the respondents, as well as with the size of the locality where respondents live (rc =0.30; p<0.001). It was revealed that men (47.2±3.6%), persons over 50 years of age (47.5±5.0%) and residents of small towns with a population of 3,000 to 10,0000 residents (47.7±4.3%) are more skeptical about this issue. The majority of respondents have a moderate level of epidemiological vigilance – 299 (54.4±2.1%); this means that a person emphasizes the seriousness and real threats of coronavirus infection, perceives and adheres to the adopted measures, but with certain hesitations. A high level of epidemiological vigilance characterized by confidence in the real situation and the adopted measures is found in 119 (21.6±1.8%) respondents. A low level of epidemiological vigilance is in 121 (22.0±1.8%) respondents. Only 11 people (2.0±0.6%) have the overall sum of points, which indicates the absence of epidemiological vigilance.
- [COVID 19: what you need to know about the coronavirus pandemic] Klinichna imunolohiia. Alerholohiia. Infektologiia. 2020;2:18-20. Ukrainian.
- Antomonov MYu. [Mathematical processing and analysis of biomedical data]. Kyiv; 2017. Russian.
- Zadorozhna VI, Vynnyk NP. [Koronavirus 2019-nCOV: new challenges to health and humanity]. Infektsiini khvoroby.2020(1):5-15. doi: https://doi.org/10.11603/1681-2727.2020.1.11091
- Mychalska SA. [Psychological features of the elderly]. Collection of Research Papers "Problems of Modern Psychology". Ukrainian. doi: https://doi.org/10.32626/2227-6246.2014-23.%25p
- [Ministry of Health of Ukraine. Business support programs]. [Internet]; (2021). Ukrainian. Available from: https://covid19.gov.ua/prohramy-pidtrymky-biznesu
- Pinchuk IY, Pishel VY, Polyvianaia MY, Yachnik YV, Virchenko VV. Occupational stress in healthcare workers during a COVID-19 pandemic. Medicni perspektivi. 2021;26(4):196-204.
- [Attitude of citizens of Ukraine to vaccination against COVID-19]. [Internet]. Kyiv: Razumkov Center; 2021 [cited 2021 Oct 23]. Ukrainian. Available from: https://razumkov.org.ua/napriamky/sotsiologichni-doslidzhennia/stavlennia-gromadian-ukrainy-do-vaktsynatsii-vid-covid19-traven-2021r
- [Plague and cholera: how Odessa experienced epidemics in different years]. [Internet]. Odessa; 2020 [cited 2021 Dec 02]. Russian. Available from: https://od.vgorode.ua/news/obschestvo/425591-chuma-y-kholera-kak-odessa-perezhyvala-epydemyy-v-raznye-hody
- Shevchenko OA, Dorohanʹ SB. [Radiothriving of the Population: Monograph]. Saarbrücken: LAP Lambert Academic Publishing; 2020. Ukrainian.
- [Chief Sanitary Inspectorate]. [Internet]. Warsaw: Service of the Republic of Poland; [cited 2021 Nov 11]. Polish. Available from: https://www.gov.pl/web/gis/stacje-sanitarno-epidemiologiczne
- Luo H, Tang QL, Shang YX, Liang SB, Yang M, Robinson N, Liu JP. Can Chinese Medicine Be Used for Prevention of Corona Virus Disease 2019 (COVID-19)? A Review of Historical Classics, Research Evidence and Current Prevention Programs. Chin J Integr Med. 2020 Apr;26(4):243-50. doi: https://doi.org/10.1007/s11655-020-3192-6
- Saracci R. Prevention in COVID-19 time: from failure to future. J Epidemiol Community Health. 2020 Sep;74(9):689-91. Epub 2020 Jun 28. PMID: 32595136; PMCID: PMC7320718.
- Tan W, Hao F, McIntyre RS, Jiang L, Jiang X, Zhang L, et al. Is returning to work during the COVID-19 pandemic stressful? A study on immediate mental health status and psychoneuroimmunity prevention measures of Chinese workforce. Brain Behav Immun. 2020 Jul;87:84-92. Epub 2020 Apr 23. doi: https://doi.org/10.1016/j.bbi.2020.04.055
- Wang J, Wang Z. Strengths, Weaknesses, Opportunities and Threats (SWOT) Analysis of China's Prevention and Control Strategy for the COVID-19 Epidemic. Int J Environ Res Public Health. 2020 Mar 26;17(7):2235. doi: https://doi.org/10.3390/ijerph17072235
- WHO: Current WHO global phase of pandemic alert: Avian Influenza A(H5N1). [Internet]. World Health Organization; [cited 2021 Nov 11]. Available from: https://www.who.int/influenza/preparedness/pandemic/h5n1phase/en/
Korshun M.M., Martіianova Y.V. Hygienic assessment of the potential risk for public health caused by groundwater and surface water pollution by pesticides of various chemical classes
Key words: pesticides, soil, water reservoirs, risk assessment
Abstract. Hygienic assessment of the potential risk for public health caused by groundwater and surface water pollution by pesticides of various chemical classes. Korshun M.M., Martіianova Y.V. The aim of the study was to compare the potential danger for human health caused by pollution in both underground and surface water sources with the fungicide pydiflumetofen and herbicides amicarbazone and bicyclopyrone in a wide range of soil-climatic conditions. Three methods were used: determination of the integral hazard vector (R); predicting the negative impact of pesticides on the health of the population by integral groundwater contamination hazard index (IGCHI), and a comprehensive risk assessment of the negative effects of pesticides on human body, when they are washed out into water (Р). It was found that the highest level of danger for public health due to possible pollution of water sources, is inherent in bicyclopyrone regardless of the soil-climatic conditions and the used assessment method (R=144.6-173.2 – very high potential hazard; IGCHI=12 points – extremely dangerous for humans, 1А class). Pydiflumetofen has a high potential integral vector hazard (R=115.8-137.5) and simultaneously by the value of the IGCHI (7-9 points) can be assessed from dangerous (2 class) to highly dangerous (1B class) for humans, depending on soil-climatic conditions. Amicarbazone by IGCHI (11 points) is extremely dangerous for humans (1A class), and at the same time by the integral vector (R=76.8-122.5) its potential danger varies from medium to high, depending on the soil-climatic conditions. Assessing the risk of the investigated pesticides, based on the comparison between potential exposure and the permissible daily intake it was concluded that the potential risk of adverse effects on public health (P) of all investigated substances is acceptable.
- Zorina OV. [Hygienic assessment of water quality in noncentralized potable water supply and improvement of legislative and regulatory framework in this field]. Naukovi dopovidi NUBiP Ukrainy. 2018;2;72. Ukrainian. Available from: http://journals.nubip.edu.ua/index.php/Dopovidi/article/view/dopovidi2018.02.003/9348.
- Sergeev SG, et al. [Indicator criteria and prediction of the hazard of groundwater pollution by herbicides based on acid esters]. Sovremennye problemy toksikolohii. 2010;2-3:76-79. Russian.
- Antonenko AM, Vavrinevych OP, Korshun MM, et al., inventors; Bogomolets National Medical University, assignee. [A way to predict the negative impact of pesticides on the public health when they get into the body with water]. Patent Ukraine 105428, MPKA61V 10/00. 2016 March 23. Ukrainian.
- Vavrinevych OP, Antonenko AM, Omelchuk ST, et al., inventors; Bogomolets National Medical University, assignee. [A method of comprehensive assessment of the risk of adverse effects of pesticides on the human body by being washed into water]. Patent Ukraine 105429, MPK A61V 10/00. 2016 March 25. Ukrainian.
- Prokopov VO. [State and Quality of Drinking Water from Centralized System of Water Supply of Ukraine under Modern Conditions (Standpoint on the Problem from the Point of View of Hygiene)]. Hihiiena naselenykh mists. 2014;64:56-67. Ukrainian.
- PPDB: Pesicide Properties Data Base. [Internet]. University of Hertfordshire. Version: July 2020; [cited 2021 Dec 7]. 2020. Available from: http://sitem.herts.ac.uk/aeru/ppdb/
- Claudia A. Spadotto. Screening method for assessing pesticide leaching potential. Pesticidas: R. Ecotoxicol. Curitiba. 2002;12:69-78. doi: https://doi.org/10.5380/pes.v12i0.3151
- Department of agriculture. [Internet]. Pydiflumentonfen. New Active Ingredient Review. CAS 1228284-64-7. EPA PC CODE 090110; 2018 June. [cited 2021 Dec 7]. Available from: https://www.mda.state.mn.us/sites/default/files/inline-files/Pydiflumetofen.pdf
- European Commission. [Internet]. Pydiflumetofen; 2019;1. [cited 2021 Dec 7]. Available from: https://echa.europa.eu/documents/10162/4d8943f7-5028-4c59-7421-8938ff1ef9c3
- Papa E, Castiglioni S, Gramatica P, et al. Screening the leaching tendency of pesticides applied in the Amu Darya Basin (Uzbekistan). Water Research. 2004;38:3485-94. doi: https://doi.org/10.1016/j.watres.2004.04.053
- Pesticides: Science and Polici. [Internet]. U.S. Environmental Protection Agency; 2016. [cited 2021 Dec 7]. Available from: https://archive.epa.gov/oppefed1/web/html/index-5.html#scigrow
- Public release summary on the evaluation of pydiflumetofen in the product Miravis Fungicide. [Internet]; 2018. [cited 2021 Dec 7]. Available from: https://apvma.gov.au/sites/default/files/publication/29011-pydiflumetofen_draft_prs-final_.pdf
- Public release summary on the evaluation of the new active bicyclopyrone in the product Talinor Herbicide. [Internet]; 2017. [cited 2021 Dec 7]. Available from: https://apvma.gov.au/sites/default/files/publication/26736-prs-bicyclopyrone-talinor-herbicide.pdf
- Public release summary on the evaluation of the new product Amitron 700WG Herbicide. [Internet]; 2018. [cited 2021 Dec 7]. Available from: https://apvma.gov.au/sites/default/files/ publication/29506-amitron_700wg_herbicide _prs.pdf
- US EPA-Pesticides; Amicarbazone: HED Human Health Risk Assessment for New Food Use Herbicide on Field Corn. [Internet]; 2005. [cited 2021 Dec 7]. Available from:https://archive.epa.gov/pesticides/chemicalsearch/chemical/foia/web/pdf/114004/114004-2005-08-10a.pdf
- Vogue PA, Kerle EA, Jenkins JJ. OSU Extension Pesticide Properties Database; National pesticide information center. [Internet]; 1994. Available from: http://npic.orst.edu/ingred/ppdmove.htm
Vinogradov O.O., Guzhva O.I. The Impact of Restrictive Measures to Prevent the Spread of COVID-19 on Students’ Lifestyle
Authors and affiliations
Key words: COVID-19, restrictive measures, lifestyle, students
Abstract. The impact of restrictive measures to prevent the spread of COVID-19 on students’ lifestyle. Vinogradov O.O., Guzhva O.I. The need to develop effective strategies to overcome the social, economic, physiological and psychological consequences of the COVID-19 pandemic requires targeted research on the impact of restrictive measures on the lifestyle of people of different ages and socio-demographic groups, including youth, as the most able and active population. The article presents the assessment results of the peculiarities of the restrictive measures impact aimed at preventing the spread of acute respiratory disease COVID-19 on the territory of Ukraine on the lifestyle of student youth. The assessment of the general attitude of the respondents to the COVID-19 pandemic, as well as the peculiarities of the lifestyle before and after the introduction of restrictive measures, was conducted by interviewing students using a specially designed questionnaire. The survey involved 86 people (58.1% – women, 41.9% – men) aged 17 to 35. The majority of respondents (79.1%) were urban residents and had a household size ranging from 2 to 4 people (2.7±0.15 people). The results of the survey showed that the majority of students consider COVID-19 a dangerous infection (48.8%), feel anxiety / worry about their own health (58.1%) and the health of their loved ones (83.7%), support the establishment of quarantine (53.5%) and adhere to the recommended preventive measures (97.7%). The majority of students observe the statistics of COVID-19 in Ukraine (79.1%) and the world (81.4%). The most authoritative sources of information for the majority of respondents are official international (53.5%) and official Ukrainian sources (39.5%); however, a significant proportion of respondents also trust unofficial sources of information (23.3%). Most students do not adhere to the regime of self-isolation (51.2%), every day going outside their place of residence, daily contact with an average of 2.7±0.19 people. After the introduction of restrictive measures, the distribution of time for different types of activity has changed. There was a significant increase in time spent by students watching movies and TV series and a decrease in time for walking (p <0.05). The duration of sleep was not changed after the introduction of restrictive measures, however, the time frame of sleep shifted by about an hour – respondents began to fall asleep later on average and wake up later.
- Abbott ML. Using Statistics in the Social and Health Sciences with SPSS and Excel. John Wiley and Sons Ltd.; 2016. doi: https://doi.org/10.1002/9781119121077
- Bedford J, Enria D, Giesecke J, Heymann DL, et al. COVID-19: towards controlling of a pandemic. The Lancet. 2020;395(10229):1015-8. doi: https://doi.org/10.1016/S0140-6736(20)30673-5
- Burdorf A, Porru F, Rugulies R. The COVID-19 (Coronavirus) pandemic: consequences for occupational health. Scand. J. Work Environ Health. 2020;46(3):229-30. doi: https://doi.org/10.5271/sjweh.3893
- Cucinotta D, Vanelli M. WHO Declares COVID-19 a Pandemic. Acta Bio-medica: Atenei Parmensis. 2020;91(1);157-60. doi: https://doi.org/10.23750/abm.v91i1.9397
- Divisi D, Di Leonardo G, Zaccagna G, Crisci R. Basic statistics with Microsoft Excel: a review. J. Thorac. Dis. 2017;9(6):1734-40. doi: https://doi.org/10.21037/jtd.2017.05.81
- Lipsitch M, Swerdlow DL, Finelli L. Defining the Epidemiology of Covid-19 – Studies Needed. N Engl J Med. 2020;382:1194-6. doi: https://doi.org/10.1056/NEJMp2002125
- Rothan HA, Byrareddy SN. The epidemiology and pathogenesis of coronavirus disease (COVID-19) outbreak. Journal of Autoimmunity. 2020;109:102433. doi: https://doi.org/10.1016/j.jaut.2020.102433
- Soloviov SO, Dziublyk IV, Mintser OP. [Prediction Model of Coronavirus Infection COVID-19 Epidemic Process in Ukraine]. Medical Informatics and Engineering. 2020;2:70-78. Ukrainian. doi: https://doi.org/10.11603/mie.1996-1960.2020.2.11176
- Velavan TP, Meyer CG. The COVID‐19 epidemic. Trop Med Int Health. 2020;25(3):278-80. doi: https://doi.org/10.1111/tmi.13383
- Sohrabi C, Alsafi Z, O’Neill N, et al. World Health Organization declares global emergency: A review of the 2019 novel coronavirus (COVID-19). International Journal of Surgery. 2020;76:71-76. doi: https://doi.org/10.1016/j.ijsu.2020.02.034
Kyselov S.М., Nazarenko O.V. The impact of systemic changes on quality of care providing in acute myocardial infarction in Ukraine
Key words: acute myocardial infarction, percutaneous coronary interventions, reperfusion therapy, quality of care
Abstract. The impact of systemic changes on quality of care providing in acute myocardial infarction in Ukraine. Kyselov S.М., Nazarenko O.V. For the past 5 years, the system of providing medical care to patients with acute myocardial infarction (AMI) has radically changed in Ukraine. The accession of our country to the European initiative "Stent for Life" contributed to the creation of the national reperfusion network. It ensured the wide availability of medical care for patients with AMI due to the fastest delivery of patients to clinics that provide a 24-hour emergency coronary artery stenting. Nowadays in Ukraine, 42 reperfusion centers are successfully operating 24/7/365 care delivery and more than 50% of patients with AMI undergo primary percutaneous coronary intervention (PCI). Almost 70% of patients are delivered to clinics within the first 6 hours after the onset of AMI symptoms, which corresponds to the obligatory “therapeutic window” for emergency PCI. The average number of primary PCI performed in AMI increased by 4 times in 2018 compared to 2012, reaching 286 procedures per 1 million population. The structure of reperfusion therapy has qualitatively changed in 2016. The total number of reperfusion procedures increased due to a decrease of thrombolytic therapy cases and an increase in the frequency of using the most effective method – primary coronary stenting in patients with STEMI. Systemic changes in the structure of medical care providing to patients with AMI contributed to a decrease in hospital lethality in patients with AMI - from 14,1% in 2012 to 13,81% in 2019.
- [Changes to the Handbook of qualification characteristics of employees' professions]. [Internet]. Kyiv: Ministry of Health of Ukraine; 2019 [cited 2021 Feb 9]. Ukrainian. Available from: https://moz.gov.ua/uploads/3/15117-dn_20191127_2347_dod.pdf
- gov.ua [Internet]. Kyiv: Ministry of Health of Ukraine; 2021 [cited 2021 Feb 9]. Ukrainian. Available from: https://moz.gov.ua/
- Sokolov MYu. [Registry of Percutaneous Coronary Interventions: Extended Comparative Analysis 2014-2015 Regional reperfusion networks in Ukraine – dynamics of development]. Sertse i sudyny. 2016;3:14-34. Russian.
- Sokolov MYu. [Registry of percutaneous coronary interventions: an extended comparative analysis of the results of 2016. Reperfusion paradox in Ukraine]. Sertse i sudyny. 2017;3:14-31. Russian.
- Sokolov MYu. [Register of percutaneous coronary interventions. Changes for 2015-2018 – random burst of activity or systemic transformations]. Sertse i sudyny. 2019;3:12-33. Russian. doi: http://doi.org/10.30978/HV2019-3-12
- [Statistical data]. [Internet]. Kyiv: Center of Medical Statistics of the Ministry of Health of Ukraine; 2021 [cited 2021 Feb 9]. Ukrainian. Available from http://medstat.gov.ua/ukr/statdan.html
- [Unified clinical protocol of emergency, primary, secondary (specialized) and tertiary (highly specialized) medical care and medical rehabilitation of acute coronary syndrome with ST segment elevation. Order of the Ministry of Health of Ukraine dated July 2, 2014 No. 455]. [Internet]. (2014). Ukrainian. Available from: https://www.dec.gov.ua/mtd/gostryj-koronarnyj-syndrom-z-elevacziyeyu-segmenta-st
- Jneid H, Addison D, Bhatt DL, Fonarow GC, Gokak S, Grady KL, et al. 2017 AHA/ACC Clinical Performance and Quality Measures for Adults With ST-Elevation and Non-ST-Elevation Myocardial Infarction: A Report of the American College of Cardiology/American Heart Association Task Force on Performance Measures. J Am Coll Cardiol. 2017 Oct 17;70(16):2048-90. doi: https://doi.org/10.1016/j.jacc.2017.06.032
- Ibanez B, James S, Agewall S, Antunes MJ, Bucciarelli-Ducci C, Bueno H, et al. ESC Scientific Document Group. 2017 ESC Guidelines for the management of acute myocardial infarction in patients presenting with ST-segment elevation: The Task Force for the management of acute myocardial infarction in patients presenting with ST-segment elevation of the European Society of Cardiology (ESC). Eur Heart J. 2018 Jan 7;39(2):119-77. doi: https://doi.org/10.1093/eurheartj/ehx393
- Collet JP, Thiele H, Barbato E, Barthélémy O, Bauersachs J, Bhatt DL, et al. ESC Scientific Document Group. 2020 ESC Guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation. Eur Heart J. 2020 Aug 29:ehaa575. doi: https://doi.org/10.1093/eurheartj/ehaa575
- Neumann FJ, Sousa-Uva M, Ahlsson A, Alfonso F, Banning AP, Benedetto U, et al. ESC Scientific Document Group. 2018 ESC/EACTS Guidelines on myocardial revascularization. Eur Heart J. 2019 Jan 7;40(2):87-165. doi: https://doi.org/10.1093/eurheartj/ehy394
- Kasza J, Wolfe R. Interpretation of commonly used statistical regression models. Respirology. 2014 Jan;19(1):14-21.PMID: 24372634. doi: https://doi.org/10.1111/resp.12221
- Hall M, Laut K, Dondo TB, Alabas OA, Brogan RA, Gutacker N, et al. National Institute for Cardiovascular Outcomes Research (NICOR). Patient and hospital determinants of primary percutaneous coronary intervention in England, 2003-2013. Heart. 2016 Feb 15;102(4):313-9. doi: https://doi.org/10.1136/heartjnl-2015-308616
- Kurt Huber, Bernard J. Gersh, Patrick Goldstein, Christopher B. Granger, Paul W. Armstrong, The organization, function, and outcomes of ST-elevation myocardial infarction networks worldwide: current state, unmet needs and future directions, Eur Heart J. 2014 June 14;35(23):1526-32. doi: https://doi.org/10.1093/eurheartj/ehu125
Naumenko L.Y., Zub T.О., Mametyev A.О. Place of injuries of elbow joint in the structure of primary permanent disability among Ukrainian population
Authors and affiliations
Naumenko L.Y. Dnipro State Medical University, Dnipro, Ukraine
Mametyev A.О. Dnipro State Medical University, Dnipro, Ukraine
Key words: elbow joint, consequences of injuries, primary permanent disability in Ukraine, population study
Abstract. Place of injuries of elbow joint in the structure of primary permanent disability among Ukrainian population. Naumenko L.Y., Zub T.О., Mametyev A.О. Despite the rapid development of orthopedics consequences of elbow injuries have a significant proportion of unsatisfactory treatment results until now. Owing to comprehend the sources of disability doctors could determine drawbacks of treatment and regulate rehabilitation program for patients with elbow injuries better. The purpose of the study was to investigate the structure of primary permanent disability due to elbow injuries among the Ukrainian population. The study was made on annual reports of Regional centers of medical and social expertise and the Center of medical and social expertise of the city of Kiev for 2018. During the reporting period a disability group due to upper extremity injuries was established totally for 1 211 patients in age over 18 years, among them 195 people had consequences of elbow traumas. That caused an intensive prevalence rate of 6.22 cases per 1 million of adults. Male patients (70.8%) and patients in working age (94.9%) prevailed among people with disability. 10.8% of patients were determined as people with disability without a revision period after the primary examination on medical and social expertise commission. The causes of primary permanent disability were domestic injuries (90.8%), occupational injuries (5.0%), injures during military service and battle injuries (2.1%) and disability since the childhood (2.1%). Structure according to disability groups showed the next distribution: 90.3% – the 3rd group, 9.2% – the 2nd group and 0.5% – the 1st group. Consequences of elbow injuries which led to primary permanent disability were caused by bone fractures (61.5%), elbow contractures and elbow ankyloses (19.0%), injuries of an ulnar nerve (8.7%), traumatic amputation at the elbow level (6.2%), forearm dislocations (3.1%), open wounds of elbow (1.0%), elbow ligaments ruptures (0.5%). Patients registered as disabled with a revision period during the first examination on commission had sufficient rehabilitation potential for restoration of elbow joint function and one of the upper limb in total.
- Loskutov OE, Domanskyi AN, Zherdev II, Lushnya SL. [Analysis of the results of surgical treatment for distal humerus fractures]. Trauma. 2019;20(1):23-27. Russian.
- Ekşioğlu M. Prediction equations for permanent impairment of the upper extremity due to the loss of range of motion. Work.2016;53:409-20. doi: https://doi.org/10.3233/WOR-152144
- Hong CC, Nashi N, Hey HW, Chee YH, Murphy D. Clinically relevant heterotopic ossification after elbow fracture surgery: A risk factors study. Orthopaedics & Traumatology: Surgery & Research. 2015;101(2):209-213. doi: https://doi.org/10.1016/j.otsr.2014.10.021
- Oosterwijk AM, Nieuwenhuis MK, Schouten HJ, van der Schans CP, Mouton LJ. Rating scales for shoulder and elbow range of motion impairment: Call for a functional approach. PLOS ONE 2018;13(8):e0200710. doi: https://doi.org/10.1371/journal.pone.0200710
- Riffenburgh RH, Gillen DL. Statistics in Medicine: Fourth Edition. Academic Press is an imprint of Elsevier; 2020. Available from: https://www.sciencedirect.com/book/9780128153284/statistics-in-medicine
- Perepelychnaya RY, editor. [The main indices of permanent disability and activity of medical and social expertise commissions in Ukraine during 2018: informational and analytic handbook]. Dnipro: Accent PP; 2019. Ukrainian. Available from: http://ndimspi.com/arkhyv/
- Naumenko LYu, Ipatov AV, Zub TO, Mametyev AO. [State of disability due to upper extremity traumas in Ukraine in 2017]. Trauma. 2018;19(4):9-14. Ukrainian. doi: http://dx.doi.org/10.22141/1608-1706.4.19.2018.142100
- Naumenko L, Borisova I, Berezovskyi V, Fesenko H, Zub T, Chub D. [Criteria of vital activity lesions as a methodological basis for determining disability]. Medicni perspektivi. 2017;23(2 Pt 1):32-36. Ukrainian. doi: https://doi.org/10.26641/2307-0404.2018.2(part1).129512
- Woo A, Bakri K, Moran SL. Management of ulnar nerve injuries. The Journal of Hand Surgery. 2015;40(1):173-81. doi: https://doi.org/10.1016/j.jhsa.2014.04.038
- Merad M, de Montalivet É, Touillet A, Martinet N, Roby-Brami A, Jarrassé N. Can we achieve intuitive prosthetic elbow control based on healthy upper limb motor strategies? Front. Neurorobot. 2018;12:1. doi: https://doi.org/10.3389/fnbot.2018.00001
- National Academies of Sciences, Engineering, and Medicine. The Promise of Assistive Technology to Enhance Activity and Work Participation. Upper-Extremity Prostheses. Washington: The National Academies Press; 2017;(Pt 4).
- Pierrie SN, Gaston RG, Loeffler BJ. Current Concepts in Upper-Extremity Amputation. The Journal of Hand Surgery. 2018;43(7):657-67. doi: https://doi.org/10.1016/j.jhsa.2018.03.053
- Mellema JJ, Lindenhovius AL, Jupiter JB. The posttraumatic stiff elbow: an update. Curr Rev Musculoskelet Med. 2016;9(2):190-8. doi: https://doi.org/10.1007/s12178-016-9336-9
Franchuk V.V., Mikhaylichenko B.V. Methodology of the performance of forensic medical examination in cases of medical malpractice litigation
Key words: forensic-medical examination, medical malpractice, medical errors, methodology of the performance of forensic medical examination
Abstract. Methodology of the performance of forensic medical examination in cases of medical malpractice litigation. Franchuk V.V., Mikhaylichenko B.V. Forensic medical examination is the main source of evidence in criminal cases related to defects in the provision of medical care. Despite the implementation in Ukraine of the new Criminal Procedural Code and the reform of the law enforcement system, this type of expert activity is not sufficiently regulated up to now. Development of an unified methodology for the organization and conduction of forensic medical examinations in cases of medical malpractice litigation is the goal of the research. On the basis of national legislation, 135 Court decisions and 350 case materials regarding the medical practitioners involved in negligence were investigated, algorithm of the expert commission’s activity is created. The algorithm consists of four sequential stages: preparatory, organizational and informational, analytical and evaluative and final. An acquaintance with the case materials takes place at the preparatory stage. The following problematic issues are normally solved during the 2nd stage of the algorithm: the personnel of the expert commission, an appointed rapporteur, the main disease or injury the claimed patient suffered, the literature data and normative documents that govern the provision of medical care in a particular case. At the analytical and evaluative stage, the features of the pathological process in the victim are clarified, the admitted defects of medical care are established with their expanded expert characteristics. At the final stage, the expert commission draws up an "expert conclusion", in which it states the negative consequences of an admitted defect in medical care, establishes a causal relationship and makes a reasonable conclusion about the main reason for the development of an unfavorable outcome for the patient.
- Basysta IV. [Conducting examination in criminal proceedings: current realities of legislative understanding]. Kryminalistyka i sudova ekspertyza. 2018;63(I):144-53. Ukrainian. Available from: http://nbuv.gov.ua/UJRN/krise_2018_63(1)__18
- [Instructions on conducting a forensic medical examination. Approved by order of the Ministry of Health of Ukraine of 1995, Pub. No. 6]. (1995). Ukrainian. Available from: http://zakon.rada.gov.ua/laws/show/z0254–95
- Prosecutor General’s Office of Ukraine. [Internet]. Kyiv; [cited: 2021 Dec 11]. Ukrainian. Available from: https://www.gp.gov.ua/ua/stst2011
- [Rules for conducting commission forensic medical examinations in the bureau of forensic medical examination. Approved by order of the Ministry of Health of Ukraine of 1995, Pub. No. 6]. (1995). [cited: 2021 Dec 11]. Ukrainian. Available from: http://zakon3.rada.gov.ua/laws/show/z0256–95
- Holubovych LL, Olkhovskyi VO, Holubovych AL, et al. [Project of rules for conducting forensic medical expertise (to the question of necessity of clarification and optimization of specific provisions)]. Medytsyna sohodni i zavtra. 2017;1(74):85-90. Ukrainian. Available from: http://repo.knmu.edu.ua/bitstream/123456789/19043/1/MSZ-2017-01.pdf
- Khyzhniak VV, Olkhovskyi VO, Morhun AO, et al. [Forensic and medical support of "medical cases"]. Teoriia ta praktyka sudovoi ekspertyzy i kryminalistyky. 201;18:558-67. Ukrainian. doi: https://doi.org/10.32353/khrife.2018.65
- Franchuk VV. [The forensic medical aspects of the inappropriate medical care in the modern-day Ukraine]. Sudebno-meditsinskaya ekspertiza. 2018;2(61):48-52. Russian. doi: https://doi.org/10.17116/sudmed201861248-52
- Kozan N, Voloshynovych V, Kotsyubynska Y et al. Analysis of Commission Forensic-medical Examinations performed in Cases Concerning the Responsibility of Surgical Professional Doctors. Galician med. j. 2020;27(2):E2020210. doi: https://doi.org/10.21802/gmj.2020.2.10
A CASE FROM PRACTICE
Lytvyn K.Yu., Mavrutenkov V.V., Yakunina О.M., Chykarenko Z.O., Bilokon O.O., Turchyn M.O. Guillain-Barré syndrome as an early complication of a new coronavirus infection SARS-CoV-2 (clinical case)
Authors and affiliations
Mavrutenkov V.V. Dnipro State Medical University, Dnipro, Ukraine
Yakunina О.M. Dnipro State Medical University, Dnipro, Ukraine
Chykarenko Z.O. Dnipro State Medical University, Dnipro, Ukraine
Bilokon O.O. Dnipro State Medical University, Dnipro, Ukraine
Turchyn M.O. Municipal non-profit enterprise "City Clinical Hospital No. 21 named after prof. E.G.Popkova" DCC, Dnipro, Ukraine
Key words: Guillain-Barré syndrome, coronavirus infection, polyneuropathy
Abstract. Guillain-Barré syndrome as an early complication of a new coronavirus infection SARS-CoV-2 (clinical case). Lytvyn K.Yu., Mavrutenkov V.V., Yakunina О.M., Chykarenko Z.O., Bilokon O.O., Turchyn M.O. The new strain of coronavirus SARS CoV-2 can affect any organ and system of the body. The pathogenesis of these lesions is due to both direct damage to body cells by the virus and the development of immunopathological reactions that can lead to demyelinating diseases of the nervous system. The article presents a clinical case of the development of Guillain-Barré syndrome associated with coronavirus disease in a 71-year-old man who developed after infection with a new strain of SARS CoV-2 virus. The man was hospitalized on the seventh day of the disease with complaints of unproductive cough, weakness, fever in the range of 37.5-38.7°C, shortness of breath during exercise. The clinical diagnosis was confirmed by the presence of SARS CoV-2 RNA in the nasopharyngeal secretion. According to digital radiography, the presence of interstitial pneumonia was determined. Against the background of treatment, the condition gradually improved and on the 14th day after the onset of the disease, a negative PCR result (SARS CoV-2 (-) RNA) was obtained. However, on the 16th day of hospital stay (23-24th days of the disease) he was diagnosed with polyneuropathy (Guillain-Barré syndrome), severe tetraparesis. Despite the therapy, the condition gradually deteriorated due to the progression of polyneuropathy. On the 9th day after the onset of neurological symptoms (25th days of illness), on the background of severe neurological deficits, the signs of respiratory and cardiovascular insufficiency developed, which led to the death of the patient. It has been shown that the course of Guillain-Barré syndrome, which developed after infection with a new strain of SARS CoV-2 virus, in this case has a severe course and lethal outcome of the disease. It is necessary to look for clinical predictors that would predict the occurrence of neurological complications in patients with coronavirus disease.
- Alberti P, Beretta S, Piatti M, Karantzoulis A, Piatti ML, Santoro P. et al. Guillain-Barré syndrome related to COVID-19 infection. Neurology-Neuroimmunology Neuroinflammation. 2020;7(4). doi: https://doi.org/10.1212/NXI.0000000000000741
- Chung A, Deimling M. Guillain-Barré Syndrome. Pediatrics in review. 2018;39(1):53-54. doi: https://doi.org/10.1542/pir.2017-0189
- Doets AY, Verboon C, Van Den Berg B, Harbo T, Cornblath DR, Willison HJ, et al. Regional variation of Guillain-Barré syndrome. Brain. 2018;141(10):2866-77. Available from: https://academic.oup.com/brain/article/141/10/2866/5104936?login=true
- Donofrio PD. Guillain-Barré Syndrome. CONTINUUM: Lifelong Learning in Neurology. 2017;23(5):1295-309. doi: https://doi.org/10.1212/CON.0000000000000513
- Esposito S, Longo MR. Guillain–barré syndrome. Autoimmunity reviews. 2017;16(1):96-101. doi: https://doi.org/10.1016/j.autrev.2016.09.022
- Mahecha MP, Ojeda E, Vega DA, Sarmiento-Monroy JC, et al. Guillain-Barré syndrome in Colombia: where do we stand now? Immunologic research. 2017;65(1):72-81.
- Padroni M, Mastrangelo V, Asioli GM, Pavolucci L, Abu-Rumeileh S, Piscaglia MG., et al. Guillain-Barré syndrome following COVID-19: new infection, old complication?. Journal of neurology. 2020;267(7):1877-9. doi: https://doi.org/10.1007/s00415-020-09849-6
- Rahimi K. Guillain-Barre syndrome during COVID-19 pandemic: an overview of the reports. Neurological Sciences.2020;41(11):3149-315. doi: https://doi.org/10.1007/s10072-020-04693-y
- Sedaghat Z, Karimi N. Guillain Barre syndrome associated with COVID-19 infection: a case report. Journal of Clinical Neuroscience. 2020;76:233-5. doi: https://doi.org/10.1016/j.jocn.2020.04.062
- Shahrizaila N, Lehmann HC, Kuwabara S. Guillain-Barré syndrome. The 2021. doi: https://doi.org/10.1016/S0140-6736(21)00517-1
- Sheikh KA. Guillain-Barré Syndrome. CONTINUUM: Lifelong Learning in Neurology. 2020;26(5):1184-204. doi: https://doi.org/10.1212/CON.0000000000000929
- Willison HJ, Jacobs BC, van Doorn PA. Guillain-barre syndrome. The Lancet. 2016;388(N10045):717-27. doi: https://doi.org/10.1016/S0140-6736(16)00339-1
- Zhao H, Shen D, Liu J, Chen S, Toscano G, Palmerini F, et al. Guillain-Barre syndrome associated with SARS-CoV-2 infection: causality or coincidence? Lancet Neurol. 2020;19(5):383-4. doi: https://doi.org/10.1016/S1474-4422(20)30109-5
Sydorchuk А.S., Sоrokhan V.D., Bоhachyk N.А., Drahanush А.D., Fеldman I.V., Hrubliak L.V. Clinical case of leptospirosis in a woman against chronic alcohol intoxication
Authors and affiliations
Sydorchuk А.S. Bukovinian state medical university, Chernivtsi, Ukraine
Sоrokhan V.D. Bukovinian state medical university, Chernivtsi, Ukraine
Bоhachyk N.А. Bukovinian state medical university, Chernivtsi, Ukraine
Drahanush А.D. Regional Municipal non-profit institution "Chernivtsi Municipal Clinical Hospital", Chernivtsi, Ukraine
Fеldman I.V. Regional Municipal non-profit institution "Chernivtsi Municipal Clinical Hospital", Chernivtsi, Ukraine
Hrubliak L.V. Regional Municipal non-profit institution "Chernivtsi Municipal Clinical Hospital", Chernivtsi, Ukraine
Key words: leptospirosis, epidemic anamnesis, occupation, risk factor, alcohol intoxication, blood smears
Abstract. Clinical case of leptospirosis in a woman against chronic alcohol intoxication. Sydorchuk А.S., Sоrokhan V.D., Bоhachyk N.А., Drahanush А.D., Fеldman I.V., Hrubliak L.V. An interesting clinical case of leptospirosis infection in an elderly woman with a professional way of getting infection on the background of alcohol abuse has been described. The authors emphasized the clinical features of this bacterial zoonosis, considered aspects of differential diagnosis of leptospirosis with other common infectious diseases. It has been emphasized on the intricacies of a detailed epidemiological anamnesis taking in order to identify and evaluate biorisk factors for pathogenic leptospira contamination in a particular occupational environment. It has been pointed out the necessity of a complex laboratory-instrumental investigation in the infectious hospital, since the delay of hospitalization and adequate treatment can worsen the prognosis for a favorable outcome of the clinical case and recovery. Such specific laboratory methods for verifying leptospiras in the blood of patients as a reaction of microagglutination and lysis with a kit of leptospira and bacterioscopic examination of a smear in the dark field are of high sensitivity and specificity and therefore are widely used in clinical practice.
- Kravchuk Yu. [Assessment of the situation regarding leptospirosis in the light of socio-ecosystem analysis]. Infektsiini 2015;1:79-86. doi: https://doi.org/10.11603/1681-2727.2015.1.3866
- Zinchuk OM, Zubach OO. Vasylieva NA, editors. [Leptospirosis]. Ternopil: TDMU; 2016.
- Shlapak IP, Holubovska OA, Halushko OA. [Infusion therapy of infectious diseases: a guide for a practicing physician]. Kyiv; 2015.
- Atil A, Jeffree MS, Syed Abdul Rahim SS, Hassan MR, Lukman KA, Ahmed K. Occupational Determinants of Leptospirosis among Urban Service Workers. International journal of environmental research and public health. [Internet]. 2020;17(2):427. https://doi.org/10.3390/ijerph17020427
- Gancheva G, Karcheva M. Icterohaemorrhagic leptospirosis in patients with history of adults abuse – report of two cases. Turk J Gastroenterol. 2013;24(6):549-55.
- Goarant C. Leptospirosis: risk factors and management challenges in developing countries. Research and reports in tropical medicine. 2016;7:49-62. doi: https://doi.org/10.2147/RRTM.S102543
- Lau CL, Watson CH, Lowry JH, David MC, Craig SB, Wynwood SJ, Kama M, Nilles EJ. Human Leptospirosis Infection in Fiji: An Eco-epidemiological Approach to Identifying Risk Factors and Environmental Drivers for Transmission. PLoS neglected tropical diseases. 2016;10(1):e0004405. doi: https://doi.org/10.1371/journal.pntd.0004405
- Rajapakse S, Rodrigo C, Balaji K, Fernando SD. Atypical manifestations of leptospirosis. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2015;109(5):294-302. doi: https://doi.org/10.1093/trstmh/trv026
- Sharp TM, Rivera García B, Pérez-Padilla J, Galloway RL, Guerra M, Ryff KR, et al. Early Indicators of Fatal Leptospirosis during the 2010 Epidemic in Puerto Rico. PLoS neglected tropical diseases. 2016;10(2):e0004482. doi: https://doi.org/10.1371/journal.pntd.0004482
- Zubach O, Telegina T, Semenyshyn O, Vasiunets L, Zinchuk A. Leptospirosis in Ukraine (Lviv Oblast): Clinical and Epidemiological Features. Vector borne and zoonotic diseases (Larchmont, N.Y.). 2019;19(5):341-6. doi: https://doi.org/10.1089/vbz.2018.2375
Tkach V.Ye., Voloshynovych M.S., Romanchuk S.M., Girnyk G.Ye., Matkovska N.R., Kozak N.V. Clinical cases of Darier-White follicular dyskeratosis
Authors and affiliations
Tkach V.Ye. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Girnyk G.Ye. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Kozak N.V. Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Key words: Darier-White disease, differential diagnosis, dermoscopy, treatment
Abstract. Clinical cases of Darier-White follicular dyskeratosis. Tkach V.Ye., Voloshynovych M.S., Romanchuk S.M., Girnyk G.Ye., Matkovska N.R., Kozak N.V. Follicular dyskeratosis (Darier-White disease) is a hereditary skin disease that is extremely rare in medical practice, so errors in its diagnosis can occur. It was first described in 1889 by two scientists (independently of each other), Ferdinand-Jean Darier and James White. The disease is inherited in an autosomal dominant pattern with variable gene penetrance. The main cause of this pathological process is a mutation of the ATP2A2 gene, located in the long arm of chromosome 12. Both men and women are affected with the same probability, but clinical manifestations are more severe in males. It appears, as a rule, at the age of 20; children under 10 rarely suffer from DAR; people who are over 30 years have very little chance of contracting this disease. Clinical manifestations slowly progress up to the age of 40-50, and gradually disappear in older age. Typical rashes are follicular papules, 0.2-0.5 cm in size, yellow-brown or dark brown in color, covered with dry or oily layers in the form of horny scales. On the surface layers, black dots are visible which are hair follicle funnels. Papules are rounded, dense, flat or spherical, initially isolated, eventually merging into plaques. They are mostly localized on the skin of the limbs, trunk, chest, in the interscapular area, large folds, less often on the neck, scalp, auricles, and in the ear canals. In 20-30 percent of patients, rashes are localized on the mucous membrane of the oral cavity next to the affected skin. More than 90 percent of patients have nail abnormalities, such as erythro- and leukonychia. Other possible manifestations are fragility, splinter hemorrhages, marked subungual hyperkeratosis, which can be observed on 2-3 nails or affect all nail plates. The dermatoscopy of skin lesions is characterized by the presence of several polygonal, branched, star-shaped or rounded-oval yellow-orange-brown lumps of various sizes, surrounded by a thin whitish halo and pink small-structured areas. The prognosis for recovery is not favorable, the treatment is ineffective. Two cases of Darier-White disease are described in this article.
- Oleinyk IO, Stulii OM. [Case of Darier’s disease]. Dermatolohiia ta venerolohiia. 2019;1:42-45. Russian. doi: https://doi.org/10.33743/2308-1066-2019-1-42-45
- Ahanian T, Curman P, Leong IUS, Brismar K, Bachar-Wikstrom E, Cederlöf M, et al. Metabolic phenotype in Darier disease: A cross-sectional clinical study. Diabetology and Metabolic Syndrome 2020;12. doi: https://doi.org/10.1186/s13098-020-0520-0
- Almeida A, Lobo M de L, Moura C, Rivera I. Darier disease: first molecular study of a Portuguese family. Heliyon2019;5:e02520. doi: https://doi.org/10.1016/j.heliyon.2019.e02520
- Behera B, Kumari R, Thappa DM, Gochhait D, Ayyanar P. Linear comedonal Darier disease: A rare variant. Australasian Journal of Dermatology 2020:ajd.13468. doi: https://doi.org/10.1111/ajd.13468
- Beiu C, Giurcaneanu C, Mihai M, Popa LG, Hage R. Darier Disease – A Clinical Illustration of Its High Variable Expressivity. Cureus.2019;11(12):e6292. doi: https://doi.org/10.7759/cureus.6292
- Cederlöf M, Bergen SE, Långström N, Larsson H, Boman M, Craddock N, et al. The association between Darier disease, bipolar disorder, and schizophrenia revisited: a population-based family study. Bipolar Disorders. 2015;17:340-4. doi: https://doi.org/10.1111/bdi.12257
- Errichetti E, Stinco G. Dermoscopy in General Dermatology: A Practical Overview. Dermatology and Therapy.2016;6:471-507. doi: https://doi.org/10.1007/s13555-016-0141-6
- Flores-Terry M, García-Arpa M, Llamas-Velasco M, Mendoza-Chaparro C, Ramos-Rodríguez C. Acral Hemorrhagic Darier Disease. Actas Dermo-Sifiliograficas 2017;108:e49-52. doi: https://doi.org/10.1016/j.adengl.2017.06.003
- Lacarruba F, Boscaglia S, Nasca MR, Caltabiano R, Micali G. Grover’s disease: dermoscopy, reflectance confocal microscopy and histopathological correlation. Dermatology Practical & Conceptual. 2017;7:51-4. doi: https://doi.org/10.5826/dpc.0703a11
- Lacarrubba F, Verzì AE, Errichetti E, Stinco G, Micali G. Darier disease: Dermoscopy, confocal microscopy, and histologic correlations. Journal of the American Academy of Dermatology. 2015;73:e97-9. doi: https://doi.org/10.1016/j.jaad.2015.04.066
- Oi-Yee Li H, Colantonio S, Kanigsberg N. Treatment of Darier’s disease with oral magnesium: a case report. SAGE Open Medical Case Reports. 2018;6. doi: https://doi.org/10.1177/2050313x18795071
- Peccerillo F, Longhitano S, Ferrari B, Bigi L, Pellacani G, Odorici G. A Peculiar Case of Darier Disease in Blaschkoid Distribution. Dermatology Practical & Conceptual.2020:e2020078. doi: https://doi.org/10.5826/dpc.1004a78
- Schneider SL, Tosti A. Tips to diagnose uncommon nail disorders. Dermatologic Clinics. 2015;33:197-205. doi: https://doi.org/10.1016/j.det.2014.12.003
- Takagi A, Kamijo M, Ikeda S. Darier disease. The Journal of Dermatology. 2016;43:275-9. doi: https://doi.org/10.1111/1346-8138.13230
- Zhang XL, Zhang W, Liu Y, Hou W. Darier disease restricted to the buttocks. Journal of the European Academy of Dermatology and Venereology. 2020 Novem 01. doi: https://doi.org/10.1111/jdv.17019